临床荟萃
臨床薈萃
림상회췌
CLINICAL FOCUS
2015年
3期
290-292,296
,共4页
李龙%唐发兵%余勤
李龍%唐髮兵%餘勤
리룡%당발병%여근
肺肿瘤%受体,表皮生长因子%MAP激酶信号系统%STAT1 转录因子
肺腫瘤%受體,錶皮生長因子%MAP激酶信號繫統%STAT1 轉錄因子
폐종류%수체,표피생장인자%MAP격매신호계통%STAT1 전록인자
lung neoplasms%receptor,epidermal growth factor%MAP kinase signaling system%STAT1 transcription factor
目的：探讨 RAS／MAPK信号传导通路与肺腺癌临床病理特征以及预后的关系。方法采用免疫组织化学法和蛋白印迹法（Western blot）检测23例肺腺癌患者及9例非癌肺疾病患者组织标本中表皮生长因子受体（EGFR）、磷酸化信号转导转录因子1（pSTAT1）和磷酸化 RAS／MAPK信号通路活化蛋白（pERK1／2）的表达。结果EGFR和活化蛋白 pERK1／2在23例肺腺癌患者中表达率分别为73．9％（17／23）和65．2％（15／23），半定量分析显示肺腺癌组pERK1／2蛋白水平显著高于非癌组（1．303±0．656）％ vs （0．262±0．213）％，且pERK1／2表达与肺腺癌患者的病理分级程度和有无淋巴结转移之间差异有统计学意义（χ2＝12．049和6．626，均P ＜0．05），肺癌不同 TNM分期间 pERK1／2表达差异无统计学意义；STAT1在23例肺腺癌患者肺组织中阳性表达率为30．4％，与非癌组比较差异无统计学意义。结论 RAS／MAPK信号传导通路的激活与肺腺癌的发生、发展和转移密切相关，可作为评估肺腺癌进展的有价值的指标。
目的：探討 RAS／MAPK信號傳導通路與肺腺癌臨床病理特徵以及預後的關繫。方法採用免疫組織化學法和蛋白印跡法（Western blot）檢測23例肺腺癌患者及9例非癌肺疾病患者組織標本中錶皮生長因子受體（EGFR）、燐痠化信號轉導轉錄因子1（pSTAT1）和燐痠化 RAS／MAPK信號通路活化蛋白（pERK1／2）的錶達。結果EGFR和活化蛋白 pERK1／2在23例肺腺癌患者中錶達率分彆為73．9％（17／23）和65．2％（15／23），半定量分析顯示肺腺癌組pERK1／2蛋白水平顯著高于非癌組（1．303±0．656）％ vs （0．262±0．213）％，且pERK1／2錶達與肺腺癌患者的病理分級程度和有無淋巴結轉移之間差異有統計學意義（χ2＝12．049和6．626，均P ＜0．05），肺癌不同 TNM分期間 pERK1／2錶達差異無統計學意義；STAT1在23例肺腺癌患者肺組織中暘性錶達率為30．4％，與非癌組比較差異無統計學意義。結論 RAS／MAPK信號傳導通路的激活與肺腺癌的髮生、髮展和轉移密切相關，可作為評估肺腺癌進展的有價值的指標。
목적：탐토 RAS／MAPK신호전도통로여폐선암림상병리특정이급예후적관계。방법채용면역조직화학법화단백인적법（Western blot）검측23례폐선암환자급9례비암폐질병환자조직표본중표피생장인자수체（EGFR）、린산화신호전도전록인자1（pSTAT1）화린산화 RAS／MAPK신호통로활화단백（pERK1／2）적표체。결과EGFR화활화단백 pERK1／2재23례폐선암환자중표체솔분별위73．9％（17／23）화65．2％（15／23），반정량분석현시폐선암조pERK1／2단백수평현저고우비암조（1．303±0．656）％ vs （0．262±0．213）％，차pERK1／2표체여폐선암환자적병리분급정도화유무림파결전이지간차이유통계학의의（χ2＝12．049화6．626，균P ＜0．05），폐암불동 TNM분기간 pERK1／2표체차이무통계학의의；STAT1재23례폐선암환자폐조직중양성표체솔위30．4％，여비암조비교차이무통계학의의。결론 RAS／MAPK신호전도통로적격활여폐선암적발생、발전화전이밀절상관，가작위평고폐선암진전적유개치적지표。
ABSTRACT:Objective To investigate the role of RAS/MAPK signal transduction pathway in the clinically biological behavior of lung adenocarcinoma and its correlation with the prognosis of patients with lung adenocarcinoma. Methods The expression of epidermal growth factor receptor (EGFR ), phosphorylation of signal transcription activation transducer 1 (pSTAT1)and phosphorylation of extracellular signal-regulated kinase 1/2 (pERK1/2)in 23 cases of lung adenocarcinoma tissues and 9 cases of nonneoplastic lung tissues were determined using immunohistochemical method.Results In the adenocarcinoma group,the overexpression rates of EGFR and pERK1/2 in lung adenocarcinoma tissues were 73.9%(17/23)and 65.2%(15/23),respectively.The expression of pERK1/2 was positively correlated with pathological stages and lymph node metastasis (χ2=12.049 and 6.626,both P<0.05), but not with TNM stage.The expression rate of pSTAT1 in lung adenocarcinoma tissues was 30.4%,which was not significantly different from that in nonneoplastic lung tissues.Conclusion The activation of RAS/MAPK signal transduction pathway is closely related to the occurrence,progression and metastasis of lung adenocarcinoma.Proteins such as EGFR and pERK1/2 can be used as valuable indicators for evaluating the progress of lung adenocarcinoma.
