中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
3期
427-433
,共7页
杨迪诚%钟建%刘涛%闫策%何丹农
楊迪誠%鐘建%劉濤%閆策%何丹農
양적성%종건%류도%염책%하단농
生物材料%骨生物材料%透钙磷石骨水泥%人工骨组织修复材料%微纳米共混体系%β-磷酸三钙%盐酸万古
生物材料%骨生物材料%透鈣燐石骨水泥%人工骨組織脩複材料%微納米共混體繫%β-燐痠三鈣%鹽痠萬古
생물재료%골생물재료%투개린석골수니%인공골조직수복재료%미납미공혼체계%β-린산삼개%염산만고
Subject headings:Calcium Phosphates%Vancomycin%Nanostructures
背景：与其他磷酸钙类骨水泥相比，透钙磷石骨水泥在生物体内具有更好的生物降解能力，能被生物体较快吸收，但其在生物体内的机械性能会有所下降，同时其固化时间过快，可注射性较差。
<br> 目的：以β-磷酸三钙为主体骨水泥粉末，搭配合适的骨水泥固化液，制备新型透钙磷石骨水泥，改善其固化性能，同时观察其载药性能。
<br> 方法：以碳酸钙和磷酸氢钙为原料制备β-磷酸三钙粉末；将柠檬酸、磷酸化壳聚糖、明胶、羟丙基甲基纤维素与水混合溶解，制备骨水泥固化液，将β-磷酸三钙、一水合磷酸二氢钙的混合粉末与固化液混合制备透钙磷石骨水泥。考察透钙磷石骨水泥的微观形貌、组成成分、粒径大小及分布、固化时间、抗压强度及释药性能。
<br> 结果与结论：β-磷酸三钙为微、纳米共混体系，微米级颗粒直径为(2.24±0.38)μm，纳米级颗粒直径为(334.95±151.62) nm；透钙磷石为片状结晶，堆积较为紧密。制备的骨水泥粉末主要成分为透钙磷石，同时还存有部分未反应的β-磷酸三钙。透钙磷石骨水泥的固化时间为(6.20±1.30) min，抗压强度为(22.90±3.13) MPa。药物释放实验证明透钙磷石骨水泥具有一定的缓释能力。
揹景：與其他燐痠鈣類骨水泥相比，透鈣燐石骨水泥在生物體內具有更好的生物降解能力，能被生物體較快吸收，但其在生物體內的機械性能會有所下降，同時其固化時間過快，可註射性較差。
<br> 目的：以β-燐痠三鈣為主體骨水泥粉末，搭配閤適的骨水泥固化液，製備新型透鈣燐石骨水泥，改善其固化性能，同時觀察其載藥性能。
<br> 方法：以碳痠鈣和燐痠氫鈣為原料製備β-燐痠三鈣粉末；將檸檬痠、燐痠化殼聚糖、明膠、羥丙基甲基纖維素與水混閤溶解，製備骨水泥固化液，將β-燐痠三鈣、一水閤燐痠二氫鈣的混閤粉末與固化液混閤製備透鈣燐石骨水泥。攷察透鈣燐石骨水泥的微觀形貌、組成成分、粒徑大小及分佈、固化時間、抗壓彊度及釋藥性能。
<br> 結果與結論：β-燐痠三鈣為微、納米共混體繫，微米級顆粒直徑為(2.24±0.38)μm，納米級顆粒直徑為(334.95±151.62) nm；透鈣燐石為片狀結晶，堆積較為緊密。製備的骨水泥粉末主要成分為透鈣燐石，同時還存有部分未反應的β-燐痠三鈣。透鈣燐石骨水泥的固化時間為(6.20±1.30) min，抗壓彊度為(22.90±3.13) MPa。藥物釋放實驗證明透鈣燐石骨水泥具有一定的緩釋能力。
배경：여기타린산개류골수니상비，투개린석골수니재생물체내구유경호적생물강해능력，능피생물체교쾌흡수，단기재생물체내적궤계성능회유소하강，동시기고화시간과쾌，가주사성교차。
<br> 목적：이β-린산삼개위주체골수니분말，탑배합괄적골수니고화액，제비신형투개린석골수니，개선기고화성능，동시관찰기재약성능。
<br> 방법：이탄산개화린산경개위원료제비β-린산삼개분말；장저몽산、린산화각취당、명효、간병기갑기섬유소여수혼합용해，제비골수니고화액，장β-린산삼개、일수합린산이경개적혼합분말여고화액혼합제비투개린석골수니。고찰투개린석골수니적미관형모、조성성분、립경대소급분포、고화시간、항압강도급석약성능。
<br> 결과여결론：β-린산삼개위미、납미공혼체계，미미급과립직경위(2.24±0.38)μm，납미급과립직경위(334.95±151.62) nm；투개린석위편상결정，퇴적교위긴밀。제비적골수니분말주요성분위투개린석，동시환존유부분미반응적β-린산삼개。투개린석골수니적고화시간위(6.20±1.30) min，항압강도위(22.90±3.13) MPa。약물석방실험증명투개린석골수니구유일정적완석능력。
BACKGROUND:Compared with other calcium phosphate cemenst, brushite calcium phosphate cement has better biodegradabilityin vivo, as it can be quickly absorbed, which also causes a decrease of its mechanical characters. And it also suffers in application from its fast setting reaction and poor injectability.
<br> OBJECTIVE:To cooperate β-tricalcium phosphate used as the main part of the solid phase with appropriate liquid phase to prepare a new brushite calcium phosphate cement formulation so as to improve the solidification properties and observe the controled drug-releasing capabilities.
<br> METHODS:β-tricalcium phosphate was synthesized by calcining calcium carbonate and calcium hydrogen phosphate. The liquid phase was prepared by mixing citric acid, phosphorylated chitosan, gelation and hydroxypropyl methyl celulose in anaqueous solution. The solid phase which consisted of β-tricalcium phosphate and monocalcium phosphate was mixed with the liquid phase to acquire brushite calcium phosphate cement. Microstructure, composition, particle size and distribution, setting time, compressive strength and drug-releasing capability of the prepared brushite calcium phosphate cement were detected.
<br> RESULTS AND CONCLUSION:β-tricalcium phosphate powder was a micro/nano blending system, with diameters of (2.24±0.38) μm in micron particle and (334.95±151.62) nm in nanoscale particle. Brushite belongs to lamelae tightly accumulated. The main component of the calcium phosphate cement powder was brushite, while a part of unreactedβ-tricalcium phosphate also existed. For the brushite calcium phosphate cement, the setting time was (6.20 ±1.30) minutes and the compressive strength was (22.90±3.13) MPa. The drug releasing experiment indicated that the brushite calcium phosphate cement also has certain controled-releasing capability.
