中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
3期
416-420
,共5页
刘继光%王立峰%李慕勤%高燕%王晓伟
劉繼光%王立峰%李慕勤%高燕%王曉偉
류계광%왕립봉%리모근%고연%왕효위
生物材料%骨生物材料%微弧氧化%纯镁%纳米SiO2%纳米TiO2%成骨细胞%国家自然科学基金
生物材料%骨生物材料%微弧氧化%純鎂%納米SiO2%納米TiO2%成骨細胞%國傢自然科學基金
생물재료%골생물재료%미호양화%순미%납미SiO2%납미TiO2%성골세포%국가자연과학기금
Subject headings:Magnesium%Metal Nanoparticles%Osteoblasts
背景：微弧氧化技术可增强镁及其合金的耐腐蚀性，提高其表面生物性能。<br> 目的：为了调控医用纯镁的生物活性，在镀液中添加纳米 SiO2或纳米 TiO2对纯镁微弧氧化涂层改良，研究其对成骨细胞增殖及分化的影响。<br> 方法：将圆形镁片分为3组，其中两组分别置入含7.5 g/L纳米SiO2或4.8 g/L纳米TiO2的硅酸盐电解液中进行表面微弧氧化处理，以未作任何处理的纯镁作为对照。将第3代成骨细胞分别接种于3组试件表面，观察成骨细胞的早期形态、增殖与碱性磷酸酶活性。<br> 结果与结论：成骨细胞在纳米SiO2组、纳米TiO2组试件表面生长状态良好，轮廓清晰，呈长梭形，多角形；在对照组表面生长状态较差。CCK-8检测显示，3组细胞吸光度值与碱性磷酸酶活性随时间推移呈上升趋势，纳米SiO2组、纳米TiO2组试件接种1，3，5 d的细胞增殖活性高于对照组；纳米SiO2组、纳米TiO2组接种3，5 d的细胞碱性磷酸酶活性高于对照组。结果表明纳米SiO2或纳米TiO2微弧氧化生物涂层可促进成骨细胞增殖及成骨活性，具有良好的生物相容性。
揹景：微弧氧化技術可增彊鎂及其閤金的耐腐蝕性，提高其錶麵生物性能。<br> 目的：為瞭調控醫用純鎂的生物活性，在鍍液中添加納米 SiO2或納米 TiO2對純鎂微弧氧化塗層改良，研究其對成骨細胞增殖及分化的影響。<br> 方法：將圓形鎂片分為3組，其中兩組分彆置入含7.5 g/L納米SiO2或4.8 g/L納米TiO2的硅痠鹽電解液中進行錶麵微弧氧化處理，以未作任何處理的純鎂作為對照。將第3代成骨細胞分彆接種于3組試件錶麵，觀察成骨細胞的早期形態、增殖與堿性燐痠酶活性。<br> 結果與結論：成骨細胞在納米SiO2組、納米TiO2組試件錶麵生長狀態良好，輪廓清晰，呈長梭形，多角形；在對照組錶麵生長狀態較差。CCK-8檢測顯示，3組細胞吸光度值與堿性燐痠酶活性隨時間推移呈上升趨勢，納米SiO2組、納米TiO2組試件接種1，3，5 d的細胞增殖活性高于對照組；納米SiO2組、納米TiO2組接種3，5 d的細胞堿性燐痠酶活性高于對照組。結果錶明納米SiO2或納米TiO2微弧氧化生物塗層可促進成骨細胞增殖及成骨活性，具有良好的生物相容性。
배경：미호양화기술가증강미급기합금적내부식성，제고기표면생물성능。<br> 목적：위료조공의용순미적생물활성，재도액중첨가납미 SiO2혹납미 TiO2대순미미호양화도층개량，연구기대성골세포증식급분화적영향。<br> 방법：장원형미편분위3조，기중량조분별치입함7.5 g/L납미SiO2혹4.8 g/L납미TiO2적규산염전해액중진행표면미호양화처리，이미작임하처리적순미작위대조。장제3대성골세포분별접충우3조시건표면，관찰성골세포적조기형태、증식여감성린산매활성。<br> 결과여결론：성골세포재납미SiO2조、납미TiO2조시건표면생장상태량호，륜곽청석，정장사형，다각형；재대조조표면생장상태교차。CCK-8검측현시，3조세포흡광도치여감성린산매활성수시간추이정상승추세，납미SiO2조、납미TiO2조시건접충1，3，5 d적세포증식활성고우대조조；납미SiO2조、납미TiO2조접충3，5 d적세포감성린산매활성고우대조조。결과표명납미SiO2혹납미TiO2미호양화생물도층가촉진성골세포증식급성골활성，구유량호적생물상용성。
BACKGROUND:The micro-arc oxidation technology can enhance the corrosion resistance of magnesium and its aloys to improve the biological properties of the surface. <br> OBJECTIVE: In order to regulate the biological activity of medical pure magnesium, to modify the micro-arc oxidation coating by adding nano-SiO2 and nano-TiO2 into the plating solution, and to study the biological coating effects on osteoblast proliferation and differentiation. <br> METHODS: Round magnesium sheets were divided into three groups, respectively treated with silicate electrolyte containing 7.5 g/L nano-SiO2 (nano-SiO2 group), 4.8 g/L nano-TiO2 (nano-TiO2 group), and nothing (control group). Passage 3 osteoblasts were seeded onto the specimen surface in the three groups to observe the early morphology, proliferation and alkaline phosphatase activity of osteoblasts. <br> RESULTS AND CONCLUSION:Osteoblasts grew wel in the nano-SiO2 group and nano-TiO2 group, presenting with clear outline, and the cels were long spindle- and polygon-shaped. But in the control group, the cels grew poor in the specimen surface. Cel counting kit-8 test showed that the absorbance values and alkaline phosphatase activities were increased with time in the three groups. Compared with the control group, the proliferative activity was higher in the nano-SiO2 group and nano-TiO2 group at days 1, 3, 5 after seeding, while the activity of alkaline phosphatase was also higher in the nano-SiO2 group and nano-TiO2 group at days 3, 5 after seeding. These findings indicate the micro-arc oxidation coatings containing nano-SiO2and nano-TiO2 can promote osteoblast proliferation and osteogenetic activity, which have good biocompatibility.
