食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2015年
3期
31-34
,共4页
郑洪艳%苏宁%霍彤%赵丹%王昌涛%杨丽
鄭洪豔%囌寧%霍彤%趙丹%王昌濤%楊麗
정홍염%소저%곽동%조단%왕창도%양려
总黄酮%大孔树脂%纯化%抗脂质过氧化
總黃酮%大孔樹脂%純化%抗脂質過氧化
총황동%대공수지%순화%항지질과양화
total flavonoids%macroporous adsorption resins%purification%anti-lipid peroxidation
以静态吸附量和解析率为评价指标,对5种大孔吸附树脂对蓝莓叶黄酮的纯化效果进行比较。结果表明DM-130吸附树脂效果最好,吸附量达到71.5 mg/g,解析率达到74.41%。纯化后的黄酮纯度达到68.8%。以抗坏血酸为对照,考察蓝莓叶黄酮对油脂氧化的抑制能力,结果显示其半数抑制浓度(IC50)为78.46μg/mL,显著强于抗坏血酸(IC50为5.56 mg/mL)。
以靜態吸附量和解析率為評價指標,對5種大孔吸附樹脂對藍莓葉黃酮的純化效果進行比較。結果錶明DM-130吸附樹脂效果最好,吸附量達到71.5 mg/g,解析率達到74.41%。純化後的黃酮純度達到68.8%。以抗壞血痠為對照,攷察藍莓葉黃酮對油脂氧化的抑製能力,結果顯示其半數抑製濃度(IC50)為78.46μg/mL,顯著彊于抗壞血痠(IC50為5.56 mg/mL)。
이정태흡부량화해석솔위평개지표,대5충대공흡부수지대람매협황동적순화효과진행비교。결과표명DM-130흡부수지효과최호,흡부량체도71.5 mg/g,해석솔체도74.41%。순화후적황동순도체도68.8%。이항배혈산위대조,고찰람매협황동대유지양화적억제능력,결과현시기반수억제농도(IC50)위78.46μg/mL,현저강우항배혈산(IC50위5.56 mg/mL)。
In this study, five kinds of macroporous adsorption resins was chosen to compare their ability of flavonoids purification. The static adsorption and desorption of flavonoids is the main evaluation index. The result shows that the DM-130 macroporous adsorption resins was the best with an adsorption capability of 71.5 mg/g and a desorption rate of 74.41 %.The purity of total flavonoids reach to 68.8 %. The anti-lipid peroxidation of total flavonoids in blueberry leaves was tested with vitamin C as reference. The IC 50 of anti-lipid peroxidation is 78.46μg/mL, which is much better than vitamin C (5.56 mg/mL).