CAJ | 학술논문

采用超高液相色谱-串联质谱联用仪（UPLC-MS/MS）技术建立了同时检测蔬菜中34种农药多残留量的快速检测方法。样品经乙腈提取，浓缩定容后用QuEChERS农残净化管净化，Acquity BEH-C18超高液相色谱柱分离，进入电喷雾串联四极杆质谱进行检测，采用多反应监测（MRM）分析，对液质分离条件进行优化。结果表明34种农药在10 ng/mL～200 ng/mL范围内线性良好（r≥0.9936）。在10μg/kg～30μg/kg范围内，平均加标回收率在68.7%～116.8%之间，RSD≤12%。该方法检出限为0.001 mg/kg～0.01 mg/kg，测定结果满足蔬菜样品中多残留农药的检测要求。
채용초고액상색보-천련질보련용의（UPLC-MS/MS）기술건립료동시검측소채중34충농약다잔류량적쾌속검측방법。양품경을정제취，농축정용후용QuEChERS농잔정화관정화，Acquity BEH-C18초고액상색보주분리，진입전분무천련사겁간질보진행검측，채용다반응감측（MRM）분석，대액질분리조건진행우화。결과표명34충농약재10 ng/mL～200 ng/mL범위내선성량호（r≥0.9936）。재10μg/kg～30μg/kg범위내，평균가표회수솔재68.7%～116.8%지간，RSD≤12%。해방법검출한위0.001 mg/kg～0.01 mg/kg，측정결과만족소채양품중다잔류농약적검측요구。
An ultra performance liquid chromatography-tandem mass spectrometric method (UPLC-MS/MS) has been developed for the simultaneous determination of 34 kinds of pesticide residues and the metabolites in vegetables. The samples were extracted by acetonitrile and purificated with QuE ChERS.The UPLC analyses were performed on an acquity UPLC C18 column with gradient eluation , combined withionization in positive mode ( ESI+) and multiple react ion monitoring (MRM) mode. The linear range was 10 ng/mL-200 ng/mL, and the correlation coefficients were more than 0.993 6. The average recoveries of 34 kinds of pesticidesin spiked vegetables (10 and 30μg/kg) ranged from 68.7 %to 116.8 %and the relative standard deviation were not more than 12 %.The detection limits were 0.001 mg/kg-0.01 mg/kg .The mult-i residue analysis method can meet the requirements for imultaneous determinationof mult-i pesticides residues in vegetables.