CAJ | 학술논문

背景：前期实验中发现载淫羊藿苷壳聚糖/胶原/聚己内酯/羟基磷灰石复合支架具有良好的物理和化学性质。 　　目的：研究载淫羊藿苷壳聚糖/胶原/聚己内酯/羟基磷灰石复合支架修复兔胫骨平台骨缺损的效果。 　　方法：利用静电纺丝技术制备胶原/聚己内酯/羟基磷灰石复合支架壳层，真空干燥法制备载淫羊藿苷壳聚糖微球/胶原支架芯层，将芯层嵌入壳层后利用京尼平交联构建载药复合支架。将载淫羊藿苷壳聚糖微球置入PBS中，观察药物缓释效果。将载药复合支架与大鼠骨髓间充质干细胞共培养7 d，观察细胞黏附效果。取青紫蓝兔15只，复制兔左侧胫骨缺损模型，随机分为3组，实验组于骨缺损处植入载药复合支架，对照组植入胶原/聚己内酯/羟基磷灰石复合支架，空白对照组不植入任何材料。术后4，12，24周行X射线观察、样本大体和组织学观察。 　　结果与结论：载药复合支架具有疏松多孔结构，利于骨髓间充质干细胞的黏附、增殖；壳聚糖微球体外缓释在72 h内保持19%释放量的良好缓释效果。术后24周，实验组材料完全被新生骨组织覆盖，硬度与正常骨相近，苏木精-伊红染色观察发现有骨小梁、骨细胞和成骨细胞，但缺损区骨密度低于正常骨组织；对照组材料被纤维组织包裹，苏木精-伊红染色显示有骨小梁、骨髓细胞和纤维组织；空白对照组呈凹陷愈合，但硬度低于正常骨组织，苏木精-伊红染色可见大量骨髓细胞和骨小梁。结果表明载药壳聚糖/胶原/聚己内酯/羟基磷灰石复合支架能有效修复兔骨缺损。
배경：전기실험중발현재음양곽감각취당/효원/취기내지/간기린회석복합지가구유량호적물리화화학성질。 　　목적：연구재음양곽감각취당/효원/취기내지/간기린회석복합지가수복토경골평태골결손적효과。 　　방법：이용정전방사기술제비효원/취기내지/간기린회석복합지가각층，진공간조법제비재음양곽감각취당미구/효원지가심층，장심층감입각층후이용경니평교련구건재약복합지가。장재음양곽감각취당미구치입PBS중，관찰약물완석효과。장재약복합지가여대서골수간충질간세포공배양7 d，관찰세포점부효과。취청자람토15지，복제토좌측경골결손모형，수궤분위3조，실험조우골결손처식입재약복합지가，대조조식입효원/취기내지/간기린회석복합지가，공백대조조불식입임하재료。술후4，12，24주행X사선관찰、양본대체화조직학관찰。 　　결과여결론：재약복합지가구유소송다공결구，리우골수간충질간세포적점부、증식；각취당미구체외완석재72 h내보지19%석방량적량호완석효과。술후24주，실험조재료완전피신생골조직복개，경도여정상골상근，소목정-이홍염색관찰발현유골소량、골세포화성골세포，단결손구골밀도저우정상골조직；대조조재료피섬유조직포과，소목정-이홍염색현시유골소량、골수세포화섬유조직；공백대조조정요함유합，단경도저우정상골조직，소목정-이홍염색가견대량골수세포화골소량。결과표명재약각취당/효원/취기내지/간기린회석복합지가능유효수복토골결손。
BACKGROUND:In the early experiments, icarin chitosan/colagen/polycaprolactone/hydroxyapatite (CS/Col/PCL/HA) composite scaffold has good physical and chemical properties. OBJECTIVE: To study the effect of CS/Col/PCL/HA composite scaffolds to repair bone defects of rabbit tibia platform. METHODS:The outer of Col/PCL/HA composite scaffolds were prepared by electrospinning, and the core containing chitosan microspheres/colagen was made by vacuum freeze-drying process. Then the core was implanted into the outer to construct the drug-loading composite scaffold by genipin crosslinking. The microspheres carrying icarin chitosan were immersed into PBS to observe the slow-release effect. The composite scaffold was used to culture rat bone marrow mesenchymal stem cels for 7 days to observe cel adhesion. Fifteen chinchila rabbits were taken to make the model of left tibia bone defect, and then randomly divided into three groups: CS/Col/PCL/HA composite scaffold group, Col/PCL/HA composite scaffold group, and blank control group with no materials. X-ray observation, gross observation, and histopathological observation were performed at 4, 12, 24 weeks after the surgery. RESULTS AND CONCLUSION: The drug-loading composite scaffold had a porous structure that was conducive to cel adhesion and proliferation. Chitosan microspheres kept a 19% drug release within 72 hoursin vitro. Twenty-four weeks after surgery, CS/Col/PCL/HA composite scaffolds were completely covered by the newly formed bone tissue, and the scaffold surfaces were smooth and had hardness close to normal bone tissue; hematoxylin-eosin staining showed bone trabecula, bone cells and osteoblasts, but X-ray images showed the bone mineral density in the defect area was slightly lower than that of the normal bone tissue. Col/PCL/HA composite scaffolds were wrapped up by fibrous tissue, and hematoxylin-eosin staining showed bone trabecula, bone marrow cels and dense fibrous tissue. In the blank control group, the hardness of defect areas was significantly lower than that of the normal bone and hematoxylin-eosin staining showed a large number of bone marrow cels and bone trabeculae. The experimental results showed that CS/Col/PCL/HA composite scaffold could repair rabbit bone defects effectively.