CAJ | 학술논문

目的：探讨糖尿病合并妊娠和妊娠期糖尿病（GDM）的SD大鼠后代中父系和母系糖脂代谢障碍的差异。方法分别把SD大鼠用STZ小剂量腹腔注射和STZ加高糖高脂饮食诱导成GDM和糖尿病合并妊娠模型，得到F1代大鼠后，分别与正常异性8周大鼠杂交产生F2代，分为对照组、GDM母系组、GDM父系组、糖尿病合并妊娠母系组与糖尿病合并妊娠父系组。测定F2代SD大鼠体质量、血糖、胰岛素、三酰甘油及瘦素水平。结果＜1周时，GDM父系组和GDM母系组体质量差异有统计学意义（P＜0．05）；8周时，糖尿病合并妊娠父系组和糖尿病合并妊娠母系组体质量差异有统计学意义（P＜0.05）；＜1周时的糖尿病合并妊娠组和8周时GDM组，父系组和母系组体质量差异均无统计学意义（P＞0．05）。8周时，糖尿病合并妊娠父系组和糖尿病合并妊娠母系组的胰岛素水平、三酰甘油水平差异有统计学意义（P＜0．05），空腹血糖水平、瘦素水平差异均无统计学意义（P＞0．05）。GDM父系组与GDM母系组的空腹血糖水平、胰岛素水平和三酰甘油水平差异有统计学意义（P＜0．05），瘦素水平差异无统计学意义（P＞0.05）。结论糖尿病大鼠的糖脂代谢障碍存在父系和母系差异，糖尿病合并妊娠性成熟期和GDM组出生时体质量差异父系组显著高于母系组。糖尿病合并妊娠母系组和GDM母系组TG水平的代谢障碍可能更明显。子代代谢障碍可能与宫内高血糖环境有关。
목적：탐토당뇨병합병임신화임신기당뇨병（GDM）적SD대서후대중부계화모계당지대사장애적차이。방법분별파SD대서용STZ소제량복강주사화STZ가고당고지음식유도성GDM화당뇨병합병임신모형，득도F1대대서후，분별여정상이성8주대서잡교산생F2대，분위대조조、GDM모계조、GDM부계조、당뇨병합병임신모계조여당뇨병합병임신부계조。측정F2대SD대서체질량、혈당、이도소、삼선감유급수소수평。결과＜1주시，GDM부계조화GDM모계조체질량차이유통계학의의（P＜0．05）；8주시，당뇨병합병임신부계조화당뇨병합병임신모계조체질량차이유통계학의의（P＜0.05）；＜1주시적당뇨병합병임신조화8주시GDM조，부계조화모계조체질량차이균무통계학의의（P＞0．05）。8주시，당뇨병합병임신부계조화당뇨병합병임신모계조적이도소수평、삼선감유수평차이유통계학의의（P＜0．05），공복혈당수평、수소수평차이균무통계학의의（P＞0．05）。GDM부계조여GDM모계조적공복혈당수평、이도소수평화삼선감유수평차이유통계학의의（P＜0．05），수소수평차이무통계학의의（P＞0.05）。결론당뇨병대서적당지대사장애존재부계화모계차이，당뇨병합병임신성성숙기화GDM조출생시체질량차이부계조현저고우모계조。당뇨병합병임신모계조화GDM모계조TG수평적대사장애가능경명현。자대대사장애가능여궁내고혈당배경유관。
Objective To investigate the paternal and maternal differences of glycolipid metabolism disorder in the offsprings of SD rats with diabetic pregnancy and gestational diabetes mellitus (GDM).Methods The GDM or diabetic pregnancy models of SD rats were in-duced by intraperitoneal injection of a small dose of STZ or STZ combined with high-sugar and high-fat diet,and then their F1 generation rats were hybridized with normal heterosexual rats of eight weeks to produce the F2 generation hybrid rats,which were divided into five groups:control group,GDM maternal group,GDM paternal group,diabetic pregnancy maternal group,and diabetic pregnancy paternal group.The body mass,levels of blood glucose,insulin,triglyceride and leptin of the F2 generation SD rats in the five groups were detected and compared.Results At <1 week after grouping,the difference in the body mass between the GDM paternal group and the GDM mater-nal group was statistically significant (P<0.05 ),while at 8 weeks,the difference in the body mass between the diabetic pregnancy paternal group and the diabetic pregnancy maternal group also became statistically significant (P<0.05 ).But in the diabetic pregnancy groups at <1 week and the GDM groups at 8 weeks,comparison with body mass between paternal and maternal groups showed no significant difference (P>0.05 ).At 8 weeks,there had significant differences in the insulin level and triglyceride level between the diabetic pregnancy paternal group and the diabetic pregnancy maternal group (P<0.05 ),with no significant differences in the fasting blood glucose level and leptin level between the two groups (P>0.05 ).The differences in the fasting blood glucose level,insulin level,and triglyceride level between the GDM paternal group and the GDM maternal group were significant (P<0.05 ),but not in the leptin level (P>0.05 ).Conclusion There is pa-ternal and maternal differences of glycolipid metabolism disorder in diabetic rats.The paternal and maternal differences of body mass in the diabetic pregnancy groups within sexual maturity period and the GDM groups within newborn period were significant.The metabolic disorder of triglyceride level in the diabetic pregnancy maternal group and the GDM maternal group may be more obvious.The offspring metabolic dis-orders may be associated with intrauterine environment of hyperglycemia.