CAJ | 학술논문

目的：探讨神经生长因子（NGF）转染的人脐带间充质干细胞（hUC- MSCs）减轻中枢神经系统功能损伤和促进中枢神经系统功能恢复的可行性。方法构建缺血、缺氧脑损伤的Wistar大鼠模型，同时分离培养hUC- MSCs，并将腺病毒介导的NGF转染hUC- MSCs。将转染成功后的hUC- MSCs移植入大鼠模型中，饲养4周后采用足印分析试验、平衡木试验分别对大鼠的运动功能进行评价。结果流式细胞仪检测提示hUC- MSCs分离培养成功，hUC- MSCs可过表达NGF蛋白。与hUC- MSCs组比较， NGF- hUC- MSCs组幼鼠右侧足印步态重复间距和平衡木通过时间均有统计学差异（均P＜0.05），左侧足印步态重复间距无统计学差异（P＞0.05）。结论 NGF联合hUC- MSCs移植能促进实验大鼠的运动功能恢复，为中枢神经系统损伤修复和并发症预防提供了新的途径。
목적：탐토신경생장인자（NGF）전염적인제대간충질간세포（hUC- MSCs）감경중추신경계통공능손상화촉진중추신경계통공능회복적가행성。방법구건결혈、결양뇌손상적Wistar대서모형，동시분리배양hUC- MSCs，병장선병독개도적NGF전염hUC- MSCs。장전염성공후적hUC- MSCs이식입대서모형중，사양4주후채용족인분석시험、평형목시험분별대대서적운동공능진행평개。결과류식세포의검측제시hUC- MSCs분리배양성공，hUC- MSCs가과표체NGF단백。여hUC- MSCs조비교， NGF- hUC- MSCs조유서우측족인보태중복간거화평형목통과시간균유통계학차이（균P＜0.05），좌측족인보태중복간거무통계학차이（P＞0.05）。결론 NGF연합hUC- MSCs이식능촉진실험대서적운동공능회복，위중추신경계통손상수복화병발증예방제공료신적도경。
Objective To investigate the effect of human umbilical cord mesenchymal stem cel s (hUC- MSCs) trans-fected with nerve growth factor (NGF) on injury recovery of central nerves in rats. Methods The isolated and cultured hUC- MSCs were transfected with adenovirus- mediated NGF gene. Ischemia hypoxia model of central nervous system was in-duced in Wistar rats. The NGF- transfected hUC- MSCs were transplanted in the model rats. The motion function of rats was eval-uated with footprint test and balance beam test. Results The transfected hUC- MSCs successful y expressed NGF protein. The footprint test and balance beam test showed that NGF- transfected hUC- MSCs promoted motor function recovery in rats with is-chemic injury of central nerves. Conclusion NGF- transfected hUC- MSCs can improve damage repair of central nerve in rats.