中医临床研究
中醫臨床研究
중의림상연구
CLINICAL JOURNAL OF CHINESE MEDICINE
2015年
3期
33-35
,共3页
脑缺血-再灌注损伤模型%SD大鼠%槲皮素-3-葡萄糖苷
腦缺血-再灌註損傷模型%SD大鼠%槲皮素-3-葡萄糖苷
뇌결혈-재관주손상모형%SD대서%곡피소-3-포도당감
目的:观察槲皮素-3-葡萄糖苷(quercitrin-3-glucoside,缩写为QG)对脑缺血-再灌注损伤大鼠的保护作用。方法:采用线栓法制备大鼠脑缺血-再灌注损伤的动物模型。通过 QG 对脑缺血-再灌注大鼠神经功能评分,病理组织学变化以及脑细胞凋亡情况的影响,探讨QG对脑缺血-再灌注损伤大鼠的保护作用及其可能的作用机制。结果:QG剂量组神经功能评分较模型组显著降低(P<0.05);TUNEL 阳性细胞数 QG 剂量组与模型组相比明显减少(P<0.05)。结论:槲皮素-3-葡萄糖苷可明显改善大鼠脑缺血-再灌注损伤,减轻缺血再灌注导致的脑水肿、氧化损伤和能量代谢障碍。
目的:觀察槲皮素-3-葡萄糖苷(quercitrin-3-glucoside,縮寫為QG)對腦缺血-再灌註損傷大鼠的保護作用。方法:採用線栓法製備大鼠腦缺血-再灌註損傷的動物模型。通過 QG 對腦缺血-再灌註大鼠神經功能評分,病理組織學變化以及腦細胞凋亡情況的影響,探討QG對腦缺血-再灌註損傷大鼠的保護作用及其可能的作用機製。結果:QG劑量組神經功能評分較模型組顯著降低(P<0.05);TUNEL 暘性細胞數 QG 劑量組與模型組相比明顯減少(P<0.05)。結論:槲皮素-3-葡萄糖苷可明顯改善大鼠腦缺血-再灌註損傷,減輕缺血再灌註導緻的腦水腫、氧化損傷和能量代謝障礙。
목적:관찰곡피소-3-포도당감(quercitrin-3-glucoside,축사위QG)대뇌결혈-재관주손상대서적보호작용。방법:채용선전법제비대서뇌결혈-재관주손상적동물모형。통과 QG 대뇌결혈-재관주대서신경공능평분,병리조직학변화이급뇌세포조망정황적영향,탐토QG대뇌결혈-재관주손상대서적보호작용급기가능적작용궤제。결과:QG제량조신경공능평분교모형조현저강저(P<0.05);TUNEL 양성세포수 QG 제량조여모형조상비명현감소(P<0.05)。결론:곡피소-3-포도당감가명현개선대서뇌결혈-재관주손상,감경결혈재관주도치적뇌수종、양화손상화능량대사장애。
Objective:The aim of This paper is mainly to study the protective effects of-3-of quercetin glucoside (Quercitrin-3-glucoside, QG) on cerebral ischemia reperfusion in rats, concerning brain tissue and brain cell apoptosis in cerebral infarction area. Methods: we prepare the MCAO model (using the suture method) 1 hour later. After 2 hours of ischemia , the rats received reperfusion administration for the following 24 hours. Then we determined the change of nerve function, infarct area, microscopic structure of brain tissue, and cell apoptosis in each group. Results: The changes of neurological function of rats: neural function score QG dose group was significantly lower than that in sham operation group(P<0.05); compared with the model group, the TUNEL positive cells decreased in control group and QG dose group(P<0.05). Conclusions:QG has a protective effect on cerebral ischemia reperfusion injury. The mechanism of this effect is probably by reducing oxidative damage and inhibiting apoptosis in cerebral ischemia reperfusion injury.