西部中医药
西部中醫藥
서부중의약
GANSU JOURNAL OF TRADITIONAL CHINESE MEDICINE
2015年
2期
23-26
,共4页
白芷%欧前胡素%异欧前胡素%星点设计%效应面法
白芷%歐前鬍素%異歐前鬍素%星點設計%效應麵法
백지%구전호소%이구전호소%성점설계%효응면법
Baizhi%imperatorin%isoimperatorin%CCD%RSM
目的：利用效应面法优选白芷的醇提取工艺参数。方法：采用HPLC方法，Waters公司的 Sun-fire C18色谱柱（150 mm×4.6mm，5μm）；流动相为甲醇-水（70∶30），等度洗脱；流速0.9 mL/min；检测波长254 nm；柱温30℃；分析时间为20分钟；进样量10μL。结果：白芷中欧前胡素的最佳提取工艺为甲醇浓度83.17%，提取时间9.15小时，甲醇用量为147.32 mL；异欧前胡素的最佳提取工艺为甲醇浓度91.83%，提取时间9.15小时，甲醇用量为112.68 mL。结论：采用星点设计与效应面法相结合的方法对实验工艺优化更方便、更准确。
目的：利用效應麵法優選白芷的醇提取工藝參數。方法：採用HPLC方法，Waters公司的 Sun-fire C18色譜柱（150 mm×4.6mm，5μm）；流動相為甲醇-水（70∶30），等度洗脫；流速0.9 mL/min；檢測波長254 nm；柱溫30℃；分析時間為20分鐘；進樣量10μL。結果：白芷中歐前鬍素的最佳提取工藝為甲醇濃度83.17%，提取時間9.15小時，甲醇用量為147.32 mL；異歐前鬍素的最佳提取工藝為甲醇濃度91.83%，提取時間9.15小時，甲醇用量為112.68 mL。結論：採用星點設計與效應麵法相結閤的方法對實驗工藝優化更方便、更準確。
목적：이용효응면법우선백지적순제취공예삼수。방법：채용HPLC방법，Waters공사적 Sun-fire C18색보주（150 mm×4.6mm，5μm）；류동상위갑순-수（70∶30），등도세탈；류속0.9 mL/min；검측파장254 nm；주온30℃；분석시간위20분종；진양량10μL。결과：백지중구전호소적최가제취공예위갑순농도83.17%，제취시간9.15소시，갑순용량위147.32 mL；이구전호소적최가제취공예위갑순농도91.83%，제취시간9.15소시，갑순용량위112.68 mL。결론：채용성점설계여효응면법상결합적방법대실험공예우화경방편、경준학。
Objective:To collect the extracting parameters of the alcohol from Baizhi (angelica dahurica) by central composite design (CCD) and response surface method (RSM). Methods:The HPLC method was performed and the detected conditions were defined, chromatography column:Waters' Sunfire C18 (150 mm×4.6mm, 5μm);mobile phase:CHOH/H2O=70/30, isocratic elution;flow rate:0.9 mL/min;detection wavelength:254 nm;column temperature:30℃;analyzing time:20 min;injection volume:10μL. Results:The best concentration of methanol for extracting the imperatorin from Baizhi was 83.17%, the extracting time was 9.15 hours and the methanol dosage was 147.32 mL,while for extracting the isoimperatorin the best concentration of methanol was 91.83%with 9.15 extract-ing hours and 112.68 mL methanol dosages. Conclusion: The method of combining CCD and RSM is easier and more accurate for optimizing the extracting process.
