西部中医药
西部中醫藥
서부중의약
GANSU JOURNAL OF TRADITIONAL CHINESE MEDICINE
2015年
2期
30-33
,共4页
款冬花%薄层色谱%款冬酮%高效液相色谱法%含量测定
款鼕花%薄層色譜%款鼕酮%高效液相色譜法%含量測定
관동화%박층색보%관동동%고효액상색보법%함량측정
KuanDongHua%TLC%tussilagone%HPLC%content determination
目的：对《中国药典》2010年版中款冬花药材的薄层色谱检测方法及款冬酮含量测定检测方法同时进行改进。方法：使用正相硅胶薄层板，通过尝试不同展开系统和检测方法，并与药典方法相对照；采用高效液相色谱法(HPLC)法测定款冬花中款冬酮的含量。色谱柱：Inertsil ODS-SP C18柱(250 mm×4.6 mm，5μm)；流动相：甲醇-水(77∶23)；流速：1.0 mL/min；检测波长：219 nm。结果：在新薄层色谱条件下，对照品斑点具有更好的分离度，无干扰，易于检测；款冬酮在0.524～1.572μg范围内具有良好的线性关系（r=0.9999，n=5)；平均回收率：98.70%，RSD=0.80%（n=9）。结论：新方法优于药典方法，可用于款冬花药材中款冬酮的定性、定量测定。
目的：對《中國藥典》2010年版中款鼕花藥材的薄層色譜檢測方法及款鼕酮含量測定檢測方法同時進行改進。方法：使用正相硅膠薄層闆，通過嘗試不同展開繫統和檢測方法，併與藥典方法相對照；採用高效液相色譜法(HPLC)法測定款鼕花中款鼕酮的含量。色譜柱：Inertsil ODS-SP C18柱(250 mm×4.6 mm，5μm)；流動相：甲醇-水(77∶23)；流速：1.0 mL/min；檢測波長：219 nm。結果：在新薄層色譜條件下，對照品斑點具有更好的分離度，無榦擾，易于檢測；款鼕酮在0.524～1.572μg範圍內具有良好的線性關繫（r=0.9999，n=5)；平均迴收率：98.70%，RSD=0.80%（n=9）。結論：新方法優于藥典方法，可用于款鼕花藥材中款鼕酮的定性、定量測定。
목적：대《중국약전》2010년판중관동화약재적박층색보검측방법급관동동함량측정검측방법동시진행개진。방법：사용정상규효박층판，통과상시불동전개계통화검측방법，병여약전방법상대조；채용고효액상색보법(HPLC)법측정관동화중관동동적함량。색보주：Inertsil ODS-SP C18주(250 mm×4.6 mm，5μm)；류동상：갑순-수(77∶23)；류속：1.0 mL/min；검측파장：219 nm。결과：재신박층색보조건하，대조품반점구유경호적분리도，무간우，역우검측；관동동재0.524～1.572μg범위내구유량호적선성관계（r=0.9999，n=5)；평균회수솔：98.70%，RSD=0.80%（n=9）。결론：신방법우우약전방법，가용우관동화약재중관동동적정성、정량측정。
Objective: To simultaneously improve the thin layer chromatography (TLC) method for Kuan-DongHua (flos farfarae) appeared in Chinese Pharmacopoeia and the determination method for tussilagone contents. Methods:The normal-phase thin-layer silica gel plate was used to detect KuanDongHua by trying different systems and determining methods, compared to the method used by Pharmacopoeia;the HPLC method was applied to test the tussilagone content. The chromatographic column:Inertsil ODS-SP C18 column (250 mm ×4.6 mm, 5μm);mo-bile phase:methanol-water=77/23;flow rate:1.0 mL/min;detection wavelength:219 nm. Results:Under the condi-tion of new thin layer chromatography, the spots of the standard substance were with better separation degree and no interference were easy to detect;the contents of the tussilagone within 0.524 ~1.572μg had a good linear relation-ship (r=0.999 9, n=5);the average recovery was 98.70%, RSD=0.80%(n=9). Conclusion:The new method is su-perior to the method used in Pharmacopoeia and can be used for the qualitative and quantitative determination of tus-silagone contents.
