临床儿科杂志
臨床兒科雜誌
림상인과잡지
2015年
2期
147-150
,共4页
肖爱菊%王团结%曹利佳%石太新%赵东菊%李培岭%任瑞娟
肖愛菊%王糰結%曹利佳%石太新%趙東菊%李培嶺%任瑞娟
초애국%왕단결%조리가%석태신%조동국%리배령%임서연
免疫性血小板减少症%DNA甲基转移酶1%DNA甲基转移酶3a%儿童
免疫性血小闆減少癥%DNA甲基轉移酶1%DNA甲基轉移酶3a%兒童
면역성혈소판감소증%DNA갑기전이매1%DNA갑기전이매3a%인동
immune thrombocytopenic purpura%DNA methyltransferase 1%DNA methyltransferase 3a%child
目的：通过检测免疫性血小板减少症(ITP)患儿外周血淋巴细胞中DNA甲基转移酶1(Dnmt1)、DNA甲基转移酶3a(Dnmt3a)mRNA的表达，探讨DNA甲基化与儿童ITP发病机制之间的联系。方法采用RT-PCR方法检测36例新诊断ITP患儿与26例健康体检儿童外周血淋巴细胞中Dnmt1、Dnmt3a mRNA的表达水平；并进行分析比较。结果新诊断ITP患儿外周血淋巴细胞中Dnmt1的mRNA表达为(3.02±0.49)，较对照组(4.58±0.52)明显降低，差异有统计学意义(t=11.95，P<0.01)；ITP患儿Dnmt3a mRNA的表达为(1.49±0.44)，较对照组(2.41±0.32)明显降低，差异有统计学意义(t=9.12，P<0.01)。结论新诊断ITP患儿可能存在DNA低甲基化，这种DNA低甲基化与儿童ITP发病机制关系密切。
目的：通過檢測免疫性血小闆減少癥(ITP)患兒外週血淋巴細胞中DNA甲基轉移酶1(Dnmt1)、DNA甲基轉移酶3a(Dnmt3a)mRNA的錶達，探討DNA甲基化與兒童ITP髮病機製之間的聯繫。方法採用RT-PCR方法檢測36例新診斷ITP患兒與26例健康體檢兒童外週血淋巴細胞中Dnmt1、Dnmt3a mRNA的錶達水平；併進行分析比較。結果新診斷ITP患兒外週血淋巴細胞中Dnmt1的mRNA錶達為(3.02±0.49)，較對照組(4.58±0.52)明顯降低，差異有統計學意義(t=11.95，P<0.01)；ITP患兒Dnmt3a mRNA的錶達為(1.49±0.44)，較對照組(2.41±0.32)明顯降低，差異有統計學意義(t=9.12，P<0.01)。結論新診斷ITP患兒可能存在DNA低甲基化，這種DNA低甲基化與兒童ITP髮病機製關繫密切。
목적：통과검측면역성혈소판감소증(ITP)환인외주혈림파세포중DNA갑기전이매1(Dnmt1)、DNA갑기전이매3a(Dnmt3a)mRNA적표체，탐토DNA갑기화여인동ITP발병궤제지간적련계。방법채용RT-PCR방법검측36례신진단ITP환인여26례건강체검인동외주혈림파세포중Dnmt1、Dnmt3a mRNA적표체수평；병진행분석비교。결과신진단ITP환인외주혈림파세포중Dnmt1적mRNA표체위(3.02±0.49)，교대조조(4.58±0.52)명현강저，차이유통계학의의(t=11.95，P<0.01)；ITP환인Dnmt3a mRNA적표체위(1.49±0.44)，교대조조(2.41±0.32)명현강저，차이유통계학의의(t=9.12，P<0.01)。결론신진단ITP환인가능존재DNA저갑기화，저충DNA저갑기화여인동ITP발병궤제관계밀절。
Objective To study the relationship between DNA methylation and pathogenesis of childhood immune thrombocytopenic purpura (ITP) by examining the expression of DNA methyltransferase 1(Dnmt1) and DNA methyltransferase 3a (Dnmt3a) mRNA in peripheral blood lymphocytes of the children with ITP. Methods Expression of Dnmt 1 and Dnmt3a mRNA in the peripheral blood lymphocytes in 36 children with newly diagnosed ITP and 26 healthy children were detected using RT-PCR. Results Dnmt1 mRNA expression in peripheral blood lymphocytes in children diagnosed with ITP was 3.02±0.49, significantly lower than 4.58±0.52 in the control group (t=11.95, P<0.001). Dnmt3a mRNA expression in peripheral blood lymphocytes in children diagnosed with ITP was 1.49±0.44, signiifcantly lower than 2.41±0.32 in the control group (t=9.12, P<0.001). Conclusions Children with newly diagnosed ITP have lower DNA methylation status in peripheral blood lymphocytes as compared to that in healthy children. The DNA methylation may play an important role in the etiology of acute ITP in children.
