临床儿科杂志
臨床兒科雜誌
림상인과잡지
2015年
2期
144-146,150
,共4页
高顺利%王立忠%刘海英%刘丹莉
高順利%王立忠%劉海英%劉丹莉
고순리%왕립충%류해영%류단리
miR-200a%急性淋巴细胞白血病%预后%儿童
miR-200a%急性淋巴細胞白血病%預後%兒童
miR-200a%급성림파세포백혈병%예후%인동
miR-200a%acute lymphoblastic leukemia%prognosis%child
目的:探讨miR-200a在儿童急性B淋巴细胞白血病中的表达及临床意义。方法收集B细胞ALL患儿45例,以非血液病患儿18例为对照组,采集骨髓标本,qRT-PCR检测各组miR-200a的表达,并分析B细胞ALL患儿中miR-200a的表达与临床指标间的相关性。结果 miR-200a在B淋巴细胞ALL患儿组中的表达为(3.9±1.4)×10-4,低于对照组(25.7±10.3)×10-4,差异有统计学意义(P<0.05)。在≥10岁B淋巴细胞ALL患儿中miR-200a的表达低于<10岁患儿,差异有统计学意义(P<0.05)。不同治疗阶段和危险度分组miR-200a表达的差异有统计学意义(P<0.05);以治疗第12周以及低危组的表达较高。结论 miR-200a低表达与儿童B细胞ALL的发生发展以及预后密切相关,可作为儿童B细胞ALL预后判断的潜在指标和治疗靶点。
目的:探討miR-200a在兒童急性B淋巴細胞白血病中的錶達及臨床意義。方法收集B細胞ALL患兒45例,以非血液病患兒18例為對照組,採集骨髓標本,qRT-PCR檢測各組miR-200a的錶達,併分析B細胞ALL患兒中miR-200a的錶達與臨床指標間的相關性。結果 miR-200a在B淋巴細胞ALL患兒組中的錶達為(3.9±1.4)×10-4,低于對照組(25.7±10.3)×10-4,差異有統計學意義(P<0.05)。在≥10歲B淋巴細胞ALL患兒中miR-200a的錶達低于<10歲患兒,差異有統計學意義(P<0.05)。不同治療階段和危險度分組miR-200a錶達的差異有統計學意義(P<0.05);以治療第12週以及低危組的錶達較高。結論 miR-200a低錶達與兒童B細胞ALL的髮生髮展以及預後密切相關,可作為兒童B細胞ALL預後判斷的潛在指標和治療靶點。
목적:탐토miR-200a재인동급성B림파세포백혈병중적표체급림상의의。방법수집B세포ALL환인45례,이비혈액병환인18례위대조조,채집골수표본,qRT-PCR검측각조miR-200a적표체,병분석B세포ALL환인중miR-200a적표체여림상지표간적상관성。결과 miR-200a재B림파세포ALL환인조중적표체위(3.9±1.4)×10-4,저우대조조(25.7±10.3)×10-4,차이유통계학의의(P<0.05)。재≥10세B림파세포ALL환인중miR-200a적표체저우<10세환인,차이유통계학의의(P<0.05)。불동치료계단화위험도분조miR-200a표체적차이유통계학의의(P<0.05);이치료제12주이급저위조적표체교고。결론 miR-200a저표체여인동B세포ALL적발생발전이급예후밀절상관,가작위인동B세포ALL예후판단적잠재지표화치료파점。
Objective To explore the expression and clinical signiifcance of microRNA-200a in childhood B-cell acute lymphoblastic leukemia (ALL). Methods Bone marrow samples were collected from 45 children with B-cell ALL and 18 chil-dren without hematology disease as control. Total RNA was acquired from bone marrow. qRT-PCR was performed to detect the expression level of miR-200a. Results The relative expression level of miR-200a in B-cell ALL group was signiifcantly lower than that in control group (P<0.05);the expression of miR-200a in children over 10 years old was signiifcantly lower than those in children under 10 years old (P<0.05);the expression of miR-200a in newly diagnosed samples was lower than those in those samples taken on Day 33 and at Week 12, respectively (P<0.05, P<0.01). In addition, the expression of miR-200a in low-risk group was higher than those in mid-risk and high-risk group, respectively (P<0.05). Conclusions Low level of miR-200a had a close correlation with the development and prognosis of childhood B cell ALL, which could be used as a potential target of thera-py and a biomarker of childhood B cell ALL in the future.