科技通报
科技通報
과기통보
BULLETIN OF SCIENCE AND TECHNOLOGY
2015年
2期
31-33
,共3页
动脉供血%调控促进模型%缺氧%负重训练
動脈供血%調控促進模型%缺氧%負重訓練
동맥공혈%조공촉진모형%결양%부중훈련
artery%the regulation to promote model%hypoxia%weight training
目的：研究磷酸化活对高原缺氧负重训练动脉供血调控促进模型,促进运动员的心肺功能，提高运动员的供血调控水平。方法：通过高原缺氧负重训练，产生大量的磷酸化活物，人体中的磷酸化活物重组质粒pET-28a-AgrC在C43(DE3)中表达为末端苏氨酸物体，通过提取人体内的血液标本，进行样本测试，对采集的运动员血液样本进行蛋白脂质体先用膜不可渗透硫醇-非荧光试剂(4-acetamido-4'-maleidylstibene-2,2'-disulphonic acid, AmdiS)标记，得到采用磷酸化活测定分析高原缺氧负重训练下的动脉供血调控促进模型结构。结果：运动员心率HR和肺活量VC显著性差异P<0.01，缺氧负重训练中最大摄氧量VO2max的值都要大于台阶实验数值，达到最大摄氧量VO2max，心率HR的显著性差异P<0.01。得到的测试组TG的心肺功能由于对比组CG1和CG2的显著差异性，进而促进动脉供血调控。结论：采用磷酸化活测定方法对高原缺氧负重训练动脉供血调控促进模型进行分析建模，对促进运动员的心肺功能，提高运动员的供血调控水平具有帮助。
目的：研究燐痠化活對高原缺氧負重訓練動脈供血調控促進模型,促進運動員的心肺功能，提高運動員的供血調控水平。方法：通過高原缺氧負重訓練，產生大量的燐痠化活物，人體中的燐痠化活物重組質粒pET-28a-AgrC在C43(DE3)中錶達為末耑囌氨痠物體，通過提取人體內的血液標本，進行樣本測試，對採集的運動員血液樣本進行蛋白脂質體先用膜不可滲透硫醇-非熒光試劑(4-acetamido-4'-maleidylstibene-2,2'-disulphonic acid, AmdiS)標記，得到採用燐痠化活測定分析高原缺氧負重訓練下的動脈供血調控促進模型結構。結果：運動員心率HR和肺活量VC顯著性差異P<0.01，缺氧負重訓練中最大攝氧量VO2max的值都要大于檯階實驗數值，達到最大攝氧量VO2max，心率HR的顯著性差異P<0.01。得到的測試組TG的心肺功能由于對比組CG1和CG2的顯著差異性，進而促進動脈供血調控。結論：採用燐痠化活測定方法對高原缺氧負重訓練動脈供血調控促進模型進行分析建模，對促進運動員的心肺功能，提高運動員的供血調控水平具有幫助。
목적：연구린산화활대고원결양부중훈련동맥공혈조공촉진모형,촉진운동원적심폐공능，제고운동원적공혈조공수평。방법：통과고원결양부중훈련，산생대량적린산화활물，인체중적린산화활물중조질립pET-28a-AgrC재C43(DE3)중표체위말단소안산물체，통과제취인체내적혈액표본，진행양본측시，대채집적운동원혈액양본진행단백지질체선용막불가삼투류순-비형광시제(4-acetamido-4'-maleidylstibene-2,2'-disulphonic acid, AmdiS)표기，득도채용린산화활측정분석고원결양부중훈련하적동맥공혈조공촉진모형결구。결과：운동원심솔HR화폐활량VC현저성차이P<0.01，결양부중훈련중최대섭양량VO2max적치도요대우태계실험수치，체도최대섭양량VO2max，심솔HR적현저성차이P<0.01。득도적측시조TG적심폐공능유우대비조CG1화CG2적현저차이성，진이촉진동맥공혈조공。결론：채용린산화활측정방법대고원결양부중훈련동맥공혈조공촉진모형진행분석건모，대촉진운동원적심폐공능，제고운동원적공혈조공수평구유방조。
Objective:To study the phosphorylation of living on plateau hypoxia weight training artery regulation to promote the model, promote the athlete's heart and lung function, improve blood supply to control the level of athletes. Methods:The plateau hypoxia weight training, the phosphorylation of living large, the body of the phosphorylation of recombinant plasmid pET-28a-AgrC in C43 was (DE3) expression in terminal threonine objects, through the blood samples from the human body, sample test, the acquisition of the athletes blood samples for protein liposome impermeable thiol non fluorescent re?agent in the first film (4-acetamido-4'-maleidylstibene-2, 2'-disulphonic acid, AmdiS) marker, obtained by phosphoryla?tion activity analysis of arterial blood supply regulation under hypoxia weight training to promote the model structure is ob?tained. Results:The heart rate and vital capacity of athletes HR VC significant difference in P<0.01, the test group TG re?ceived cardiopulmonary function because of the significant differences between group CG1 and CG2., and then promote the arterial blood supply regulation. Conclusion:The method to measure living by phosphorylation analysis of plateau hypoxia weight training artery regulation to promote the model, to promote the athlete's heart and lung function, improve blood sup?ply can help control the level of athletes.
