CAJ | 학술논문

目的：探讨茶油多种临床用药可能的药理药效基础。方法将油茶籽经两次95%的乙醇回流提取后浓缩至不含乙醇和水的提取物，取其中的茶油部分作为试验药物；用肿瘤坏死因子-α(TNF-α)处理人乳腺癌细胞系(MCF-7)细胞建立细胞炎症模型，进一步用蛋白质印迹技术观察药物处理对细胞炎症因子IκB-α表达的影响进而评价茶油是否具有抗炎活性。结果从油茶籽提取得到的茶油显著地抑制细胞中TNF-α诱导的IκB-α的降解从而抑制炎症发生。结论茶油对细胞显示良好的抗炎作用，这种特性可能是其临床应用于治疗皮肤过敏、暗疮、疥癣、小儿红屁股、以及皮肤烧烫伤等多种与炎症相关疾病的药理基础之一。
목적：탐토다유다충림상용약가능적약리약효기출。방법장유다자경량차95%적을순회류제취후농축지불함을순화수적제취물，취기중적다유부분작위시험약물；용종류배사인자-α(TNF-α)처리인유선암세포계(MCF-7)세포건립세포염증모형，진일보용단백질인적기술관찰약물처리대세포염증인자IκB-α표체적영향진이평개다유시부구유항염활성。결과종유다자제취득도적다유현저지억제세포중TNF-α유도적IκB-α적강해종이억제염증발생。결론다유대세포현시량호적항염작용，저충특성가능시기림상응용우치료피부과민、암창、개선、소인홍비고、이급피부소탕상등다충여염증상관질병적약리기출지일。
Objective To explore the possible pharmacological effect basis of usual clinical medications with camellia oil. Methods After twice 95% ethanol reflux extraction of camellia seed, the solution was concentrated to the extractive without ethanol and water. Camellia oil in the extractive was taken as study drug. Human breast adenocarcinoma cell line (MCF-7) cell was managed by tumor necrosis factor (TNF)-αto build a cell inflammation model. Influence of drug treatment on cell inflammatory cytokines IκB-αwas observed by Western blot, in order to make evaluation on whether camellia oil possesses anti-inflammatory activity. Results Camellia oil extracted from camellia seed had significantly inhibiting effect on degradation of IκB-αinduced by TNF-α, so as to suppress occurrence of inflammation. Conclusion Camellia oil has precise anti-inflammation effect on cells, and this effect may be one of its pharmacological basis for treating inflammation related diseases as skin allergy, pimples, scabies, pediatric bunda vermelha, skin burn and scald.