国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2015年
4期
464-465,469
,共3页
同型半胱氨酸%生化试剂%对比分析
同型半胱氨痠%生化試劑%對比分析
동형반광안산%생화시제%대비분석
homocysteine%biochemical reagent%comparative analysis
目的：评价4种用于同型半胱氨酸（Hcy）检测的生化试剂（A、B、C、D）在同一全自动生化分析仪器上的性能。方法对 4种 Hcy 生化试剂的重复性进行评估；将 A 试剂设为对比试剂，其余3种设为实验试剂，进行相关性分析；应用 4种生化试剂分别测定3个厂家提供的试剂配套质控品的 Hcy 值并计算相对偏差。结果4种 Hcy 生化试剂测定同份混合血清的变异系数分别为2．57％、6．12％、2．29％、3．78％；B、C、D 试剂与 A 试剂测定的相关系数分别为0．5355、0．9319、0．9577。4种 Hcy 试剂分别测定厂家提供的配套质控品 Hcy 值的相对偏差如下，A：－7．84％～13．57％，B：－19．29％～9．47％，C：－13．94％～6．67％，D：7．86％～27．37％。结论A、C、D 试剂均有较好精密度；B 试剂与 A 试剂的相关性较差，C、D 试剂与 A 试剂的相关性较好；A、C 试剂具有较好准确性。A 试剂的性能指标要优于其他3种试剂。
目的：評價4種用于同型半胱氨痠（Hcy）檢測的生化試劑（A、B、C、D）在同一全自動生化分析儀器上的性能。方法對 4種 Hcy 生化試劑的重複性進行評估；將 A 試劑設為對比試劑，其餘3種設為實驗試劑，進行相關性分析；應用 4種生化試劑分彆測定3箇廠傢提供的試劑配套質控品的 Hcy 值併計算相對偏差。結果4種 Hcy 生化試劑測定同份混閤血清的變異繫數分彆為2．57％、6．12％、2．29％、3．78％；B、C、D 試劑與 A 試劑測定的相關繫數分彆為0．5355、0．9319、0．9577。4種 Hcy 試劑分彆測定廠傢提供的配套質控品 Hcy 值的相對偏差如下，A：－7．84％～13．57％，B：－19．29％～9．47％，C：－13．94％～6．67％，D：7．86％～27．37％。結論A、C、D 試劑均有較好精密度；B 試劑與 A 試劑的相關性較差，C、D 試劑與 A 試劑的相關性較好；A、C 試劑具有較好準確性。A 試劑的性能指標要優于其他3種試劑。
목적：평개4충용우동형반광안산（Hcy）검측적생화시제（A、B、C、D）재동일전자동생화분석의기상적성능。방법대 4충 Hcy 생화시제적중복성진행평고；장 A 시제설위대비시제，기여3충설위실험시제，진행상관성분석；응용 4충생화시제분별측정3개엄가제공적시제배투질공품적 Hcy 치병계산상대편차。결과4충 Hcy 생화시제측정동빈혼합혈청적변이계수분별위2．57％、6．12％、2．29％、3．78％；B、C、D 시제여 A 시제측정적상관계수분별위0．5355、0．9319、0．9577。4충 Hcy 시제분별측정엄가제공적배투질공품 Hcy 치적상대편차여하，A：－7．84％～13．57％，B：－19．29％～9．47％，C：－13．94％～6．67％，D：7．86％～27．37％。결론A、C、D 시제균유교호정밀도；B 시제여 A 시제적상관성교차，C、D 시제여 A 시제적상관성교호；A、C 시제구유교호준학성。A 시제적성능지표요우우기타3충시제。
Objective To evaluate the performance of the four kinds of biochemical reagents (A,B,C,D)for homocysteine (Hcy)determination on the same automatic biochemical analyzer.Methods The repeatability of the four kinds of reagents were as-sessed;A reagent was set as contrast agents,the other three were set as experimental reagents,and correlation analysis were per-formed;detected the Hcy concentrations of the four quality control materials provided by the three manufacturers and calculated rel-ative deviation.Results The coefficient of variation of four kinds of Hcy biochemical agents were 2.57%,6.12%,2.29%,3.78%in the detection of the same mixed serum;the correlation coefficient of B,C,D reagents with A reagents were 0.535 5,0.931 9,and 0.957 7 respectively.Relative deviation value of Hcy concentration determination were as follows,A:- 7.84% - 13.57%,B:-19.29%-9.47%,C:-13.94%-6.67%,D:7.86%-27.37%.Conclusion A,C,D reagents have good precision;The correla-tion between B Reagent and A reagent was not good,while between C,D reagent and A reagent were good;C reagent showed good accuracy;A reagent performance is better than the other three agents.
