国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2015年
4期
449-450,453
,共3页
肺腺癌%胸腔积液%免疫细胞化学%磷酸化信号转导与转录激活因子 3
肺腺癌%胸腔積液%免疫細胞化學%燐痠化信號轉導與轉錄激活因子 3
폐선암%흉강적액%면역세포화학%린산화신호전도여전록격활인자 3
adenocarcinoma of lung%pleural effussion%immunocytochemistry%phosphorylated signal transducer and acti-vator of transcription 3
目的：探讨磷酸化信号转导与转录激活因子3（pSTAT3）蛋白表达对肺腺癌所致恶性胸腔积液的诊断价值。方法应用液基细胞学技术，对临床送检的47例胸腔积液进行检测，同时每例均利用薄层细胞涂片行免疫细胞化学染色，检测 pSTAT3蛋白的表达。结果40例胸腔积液标本经临床及医学影像学资料和（或）组织学检查证实为肺腺癌所致恶性胸腔积液，7例为良性胸腔积液。pSTAT3在肺腺癌所致恶性胸腔积液中的阳性表达率为80．0％（32／40），高于良性组的0．0％（0／7），差异有统计学意义（P ＜0．05）。pSTAT3免疫细胞化学染色联合液基细胞学检查可使恶性胸腔积液的检出率达80．0％（32／40），高于单独使用液基细胞学检查的检出率[32．5％（13／40）]，二者比较差异有统计学意义（P ＜0．05）。结论肺腺癌所致恶性胸腔积液中pSTAT3蛋白的表达明显增高，pSTAT3可作为肺腺癌所致恶性胸腔积液诊断的一个新的生物标记物，pSTAT3可能在胸腔积液的发生、发展中发挥重要作用。
目的：探討燐痠化信號轉導與轉錄激活因子3（pSTAT3）蛋白錶達對肺腺癌所緻噁性胸腔積液的診斷價值。方法應用液基細胞學技術，對臨床送檢的47例胸腔積液進行檢測，同時每例均利用薄層細胞塗片行免疫細胞化學染色，檢測 pSTAT3蛋白的錶達。結果40例胸腔積液標本經臨床及醫學影像學資料和（或）組織學檢查證實為肺腺癌所緻噁性胸腔積液，7例為良性胸腔積液。pSTAT3在肺腺癌所緻噁性胸腔積液中的暘性錶達率為80．0％（32／40），高于良性組的0．0％（0／7），差異有統計學意義（P ＜0．05）。pSTAT3免疫細胞化學染色聯閤液基細胞學檢查可使噁性胸腔積液的檢齣率達80．0％（32／40），高于單獨使用液基細胞學檢查的檢齣率[32．5％（13／40）]，二者比較差異有統計學意義（P ＜0．05）。結論肺腺癌所緻噁性胸腔積液中pSTAT3蛋白的錶達明顯增高，pSTAT3可作為肺腺癌所緻噁性胸腔積液診斷的一箇新的生物標記物，pSTAT3可能在胸腔積液的髮生、髮展中髮揮重要作用。
목적：탐토린산화신호전도여전록격활인자3（pSTAT3）단백표체대폐선암소치악성흉강적액적진단개치。방법응용액기세포학기술，대림상송검적47례흉강적액진행검측，동시매례균이용박층세포도편행면역세포화학염색，검측 pSTAT3단백적표체。결과40례흉강적액표본경림상급의학영상학자료화（혹）조직학검사증실위폐선암소치악성흉강적액，7례위량성흉강적액。pSTAT3재폐선암소치악성흉강적액중적양성표체솔위80．0％（32／40），고우량성조적0．0％（0／7），차이유통계학의의（P ＜0．05）。pSTAT3면역세포화학염색연합액기세포학검사가사악성흉강적액적검출솔체80．0％（32／40），고우단독사용액기세포학검사적검출솔[32．5％（13／40）]，이자비교차이유통계학의의（P ＜0．05）。결론폐선암소치악성흉강적액중pSTAT3단백적표체명현증고，pSTAT3가작위폐선암소치악성흉강적액진단적일개신적생물표기물，pSTAT3가능재흉강적액적발생、발전중발휘중요작용。
Objective To investigate the diagnostic value and clinical significance of phosphorylated signal transducer and activa-tor of transcription 3(pSTAT3)in malignant pleural effusion(MPE)caused by lung adenocarcinoma.Methods 47 pleural effusion samples were collected and detected by using liquid-based cytologic test (LCT).Furthermore,the expression of pSTAT3 was detec-ted by using the method of immunocytochemistry in all of the specimens.Results Among the 47 cases of pleural effusion,40 cases of MPE were caused by lung adenocarcinoma and 7 cases were benign pleural effusion,which were confirmed by clinical manifesta-tion,biopsy and imaging data.The positive expression rate of pSTAT3 was 80.0%(32/40)in MPE caused by lung adenocarcino-ma,significantly higher than benign group(P <0.05).13 cases of MPE caused by lung adenocarcinoma and 7 cases of benign pleural effusion were diagnosed with liquid-based cytologic test alone.The other 27 cases were undetermined and needed to be differentiate between adenocarcinoma and atypical mesothelial cells.However,32 cases of lung adenocarcinoma and 7 cases could be clearly diag-nosed if liquid-based cytologic test was used combined with immunocytochemistry detection of pSTAT3 expression.Only 8 cases were undetermined.Diagnosis of grey area was significantly narrowed.There was significant statistical significance between the two methods(P <0.05).Conclusion High expression of pSTAT3 is related to the MPE caused by lung adenocarcinoma.Detection of pSTAT3 expression can be used as a new method in the diagnosis of MPE caused by lung adenocarcinoma.pSTAT3 may play an important role in the development of the MPE.
