CAJ | 학술논문

目的：回顾性分析全自动血红蛋白（Hb）电泳检测的结果，并探讨 Hb 电泳在血红蛋白病筛查中的意义。方法收集2011年1月至2013年12月在该院进行血常规及全自动 Hb 电泳检测的患者临床资料，记录其平均红细胞体积（MCV）、平均红细胞血红蛋白含量（MCH）及 Hb 电泳检测结果，对疑似地中海贫血的患者进行地中海贫血基因检测，计算 Hb 电泳筛查的检出率、基因检测的符合率及各种血红蛋白病的构成比。结果共收集了12898例患者的临床资料，MCV 为（85．32±13．61）fL， MCH 为（29．87±6．44）pg，通过全自动 Hb 电泳仪共检出阳性标本1315例，其中男568例、女747例，检出率是10．19％，其中疑似α-地中海贫血的患者761例，占5．90％；疑似β-地中海贫血的患者495例，占3．84％；HbJ 患者11例，占0．08％；HbK 患者15例，占0．12％；HbG 患者9例，占0．07％；HbD 患者3例，占0．02％；HbE 患者21例，占0．16％。α-地中海贫血基因符合率80．55％，β-地中海贫血基因符合率96．77％。结论全自动 Hb 电泳检测在血红蛋白病的筛查中能发挥重要作用。
목적：회고성분석전자동혈홍단백（Hb）전영검측적결과，병탐토 Hb 전영재혈홍단백병사사중적의의。방법수집2011년1월지2013년12월재해원진행혈상규급전자동 Hb 전영검측적환자림상자료，기록기평균홍세포체적（MCV）、평균홍세포혈홍단백함량（MCH）급 Hb 전영검측결과，대의사지중해빈혈적환자진행지중해빈혈기인검측，계산 Hb 전영사사적검출솔、기인검측적부합솔급각충혈홍단백병적구성비。결과공수집료12898례환자적림상자료，MCV 위（85．32±13．61）fL， MCH 위（29．87±6．44）pg，통과전자동 Hb 전영의공검출양성표본1315례，기중남568례、녀747례，검출솔시10．19％，기중의사α-지중해빈혈적환자761례，점5．90％；의사β-지중해빈혈적환자495례，점3．84％；HbJ 환자11례，점0．08％；HbK 환자15례，점0．12％；HbG 환자9례，점0．07％；HbD 환자3례，점0．02％；HbE 환자21례，점0．16％。α-지중해빈혈기인부합솔80．55％，β-지중해빈혈기인부합솔96．77％。결론전자동 Hb 전영검측재혈홍단백병적사사중능발휘중요작용。
Objective To retrospectively analyze the result of hemoglobin(Hb)test by using full-automatic Hb electrophoresis and evaluate the its significance in hemoglobinopathy.Methods The data of patients who underwent Hb electrophoresis test and regular blood tests in the hospital from January 2011 to December 2013 were included in the study.The test results were recorded including mean corpuscular volume(MCV),mean corpuscular hemoglobin(MCH)and results of Hb electrophoresis test.Final diag-nosis were made for suspected patients by using genetic testing,then disease detection rates and gene coincidence rates and constitu-ent ratios were calculated.Results 12 898 cases were included in the study,after statistical analysis the MCV was(85.32±13.61) fL,MCH was(29.87±6.44)pg.By using automatic hemoglobin electrophoresis,1 315 cases were found to be positive,in which 568 were male,747 were female,the detection rate was 10.19%.In the 1 315 patients,there were 761 cases suspectedα-thalassemia,ac-counted for 5.90%.There were 495 cases of suspectedβ-thalassemia,accounted for 3.84%,11 patients with HbJ(0.08%),15 pa-tients with HbK(0.12%),9 patients with HbG(0.07%),3 patients with HbD(0.02%),21 patients with HbE(0.16%).The sus-pected case′s final diagnosis were made by using genetic testing,α-thalassemia gene′s coincidence rate was 80.55%,β-thalassemia gene′s coincidence rate was 96.77%.Conclusion Automatic hemoglobin electrophoresis detection is of great significance for the di-agnosis of hemoglobinopathy.