CAJ | 학술논문

目的：评价Sysmex XT-4000i全自动血细胞分析仪检测新生儿白细胞（WBC）计数及分类结果的准确性。方法采用 Sysmex XT-4000i 全自动血细胞分析仪检测与人工显微镜计数及染色分类两种方法分别对213例新生儿血液标本进行检测分析。结果仪器提示有幼稚粒细胞、原始细胞、左移三种提示中任意一种及以上的标本有112份，占52．58％（112／213），而染色镜检出现幼稚细胞或核左移的标本有91份，占42．72％（91／213）。仪器提标有有核红细胞的标本有109份，占51．17％（109／213），而染色镜检中可见有核红细胞的标本有66份，占30．99％（66／213）。仪器未能对 WBC 进行分类或分类不全的标本有11份，占5．16％（11／213）。当标本中无有核红细胞干扰或有核红细胞数量低时（≤10个／100个 WBC），仪器 WBC 计数与人工计数结果，二者差异无统计学意义（P ＞0．05），当标本中有核红细胞数量多时（＞10个／100个 WBC），仪器白细胞计数与人工计数结果，二者差异有统计学意义（P ＜0．05）。对仪器检测与手工白细胞染色分类相关分析中，中性粒细胞、淋巴细胞、单核细胞、嗜酸性粒细胞、嗜碱性粒细胞的相关系数 r 分别是：0．95、0．93、0．78、0．86、0．14。结论Sysmex XT-4000i 全自动血细胞分析仪对新生儿白细胞计数与分类存在一定误差。对新生儿血常规检测应同时进行人工镜检，并根据有核红细胞的数量进行校正，以确保为临床提供准确的血常规报告结果。
목적：평개Sysmex XT-4000i전자동혈세포분석의검측신생인백세포（WBC）계수급분류결과적준학성。방법채용 Sysmex XT-4000i 전자동혈세포분석의검측여인공현미경계수급염색분류량충방법분별대213례신생인혈액표본진행검측분석。결과의기제시유유치립세포、원시세포、좌이삼충제시중임의일충급이상적표본유112빈，점52．58％（112／213），이염색경검출현유치세포혹핵좌이적표본유91빈，점42．72％（91／213）。의 기 제 표 유 유 핵 홍 세 포 적 표 본 유109빈，점51．17％（109／213），이염색경검중가견유핵홍세포적표본유66빈，점30．99％（66／213）。의기미능대 WBC 진행분류혹분류불전적표본유11빈，점5．16％（11／213）。당표본중무유핵홍세포간우혹유핵홍세포수량저시（≤10개／100개 WBC），의기 WBC 계수여인공계수결과，이자차이무통계학의의（P ＞0．05），당표본중유핵홍세포수량다시（＞10개／100개 WBC），의기백세포계수여인공계수결과，이자차이유통계학의의（P ＜0．05）。대의기검측여수공백세포염색분류상관분석중，중성립세포、림파세포、단핵세포、기산성립세포、기감성립세포적상관계수 r 분별시：0．95、0．93、0．78、0．86、0．14。결론Sysmex XT-4000i 전자동혈세포분석의대신생인백세포계수여분류존재일정오차。대신생인혈상규검측응동시진행인공경검，병근거유핵홍세포적수량진행교정，이학보위림상제공준학적혈상규보고결과。
Objective To evaluate the accuracy of Sysmex XT-4000i automated hematology analyzer on neonatal leukocytes count and classification results.Methods 213 cases of neonatal blood specimens were analyzed respectively by Sysmex XT-4000i automated hematology analyzer and microscope counting and staining of two categories of methods.Results The instrument that immature granulocyte,primitive cell,shift to the left,three kind of tip in any one or more specimens have 112,accounted for 52.58% (112/213),and staining immature cell or shift to the left of the specimens was 91,accounted for 42.72% (91/213).The instrument to nucleated erythrocyte specimens were 109,accounting for 51.17% (109/213),and staining of nucleated erythrocyte were 66,accounting for 30.99% (66/213).Classify or classification of incomplete instrument failed to leukocytes were 11,ac-counting for 5.16% (11/213).When the specimens without nucleated erythrocytes interference or the number of nucleated eryth-rocytes is low [≤ 10/100 white blood cells(WBC)],Instruments on leukocyte count and manual counting results,there was not statistically significant differences between the two groups(P >0.05 ),when the sample number of nucleated erythrocytes (>10/100 WBC),When the specimens were nucleated erythrocyte number for a long time(>10/100 WBC),Instruments on leukocyte count and manual counting results,there was significant difference between the two groups(P <0.05).Correlation analysis on the instrument detection and manual dyeing classification of leukocytes,neutrophils,lymphocytes,monocytes,eosinophils,basophils, the correlation coefficient is respectively 0.95,0.93,0.78,0.86,0.14.Conclusion Some errors exist in Sysmex XT-4000i auto-mated hematology analyzer to count and classification of leukocytes.On neonatal blood tests should be carried out at the same time man-ual microscopy,and on the basis of nucleated erythrocytes were corrected,to ensure the clinical blood routine reporting results.