CAJ | 학술논문

目的：对 ELISA 法联合化学发光法用于特异性梅毒抗体检测的效果进行评价。方法选取2014年1月至2014年4月于该院皮肤性病科就诊患者，共628例。对患者血清采用 ELISA 法进行初筛，再将检测结果在复检标准范围内（0．5＜S／CO＜3）的样本用化学发光法进行复检，然后与梅毒螺旋体明胶凝集试验（TPPA）法平行测定的结果进行比较。结果TPPA 法检测为阳性的标本为171例；ELISA 法检测符合该科梅毒阳性判断标准的标本共162例，另外还有15例处于该科梅毒。经化学发光法进一步复检，阳性结果为9例，阴性结果为6例，并且此联合检测法得到的171例阳性结果与 TPPA 法阳性结果完全吻合。结论ELISA 法与化学发光法联合检测的结果与 TPPA 有很好的一致性。
목적：대 ELISA 법연합화학발광법용우특이성매독항체검측적효과진행평개。방법선취2014년1월지2014년4월우해원피부성병과취진환자，공628례。대환자혈청채용 ELISA 법진행초사，재장검측결과재복검표준범위내（0．5＜S／CO＜3）적양본용화학발광법진행복검，연후여매독라선체명효응집시험（TPPA）법평행측정적결과진행비교。결과TPPA 법검측위양성적표본위171례；ELISA 법검측부합해과매독양성판단표준적표본공162례，령외환유15례처우해과매독。경화학발광법진일보복검，양성결과위9례，음성결과위6례，병차차연합검측법득도적171례양성결과여 TPPA 법양성결과완전문합。결론ELISA 법여화학발광법연합검측적결과여 TPPA 유흔호적일치성。
Objective To evaluate ELISA combined with chemiluminescence in the detection of syphilis specific antibodies. Methods 628 patients who came to our hospital and treated in dermatology department were recruited in the study from January 2014 to April 2014.Patients′serum samples were detected by using ELISA firstly,and then the samples,the test rusults of which were within the recheck standard range(0.5<S/CO<3)were retested by using chemiluminescence.And then the results of parallel Treponema pallidum gelatin agglutination test(TPPA)measurement were compared with.Results There were 171 cases of TPPA positive samples detected and 162 cases meets the positive criteria for syphilis (S/CO≥3.0)in the hospital.In addition to that,15 cases of syphilis detected were within the recheck standard range.In the samples rechecked by using chemiluminescencea,there were 9 cases of positive results,and 6 cases of negative results.171 cases of positive results were detected by the combined method,which were consistent with TPPA.Conclusion The detection results of ELISA combined with chemiluminescence detection are consistent with that of TPPA.