CAJ | 학술논문

目的：探讨辅助 T 淋巴细胞（Th）亚群 Th1／Th2平衡及肠道正常菌群在儿童过敏性哮喘发病中的意义，为临床通过益生菌的早期干预来纠正 Th1／Th2平衡提供理论依据。方法ELISA 检测165例小儿过敏性哮喘患儿（哮喘组）及同期进行健康体检且合格的150例儿童（对照组）的血清 IFN-γ与 IL-4水平，同时，使用实时荧光定量 PCR 法检测粪便中双歧杆菌的数量。在应用双歧杆菌制剂调节肠道菌群6个月后再次检测上述指标，结合临床相关检查资料，对检测结果进行统计学分析。结果哮喘组初入院时血浆 IFN-γ低于对照组，差异有统计学意义（P ＜0．05），IL-4的水平高于对照组，粪便中双歧杆菌数量少于对照组，差异有统计学意义（P ＜0．05）。经过6个月的益生菌制剂的调理，哮喘组血浆 IFN-γ水平升高，IL-4水平则下降，粪便中双歧杆菌的数量增多，与初入院时比较差异均有统计学意义（P ＜0．05）。结论在儿童哮喘患儿中，肠道菌群的平衡关系到其免疫是否平衡，两者相互影响，临床应早期进行干预调节肠道菌群和免疫平衡。
목적：탐토보조 T 림파세포（Th）아군 Th1／Th2평형급장도정상균군재인동과민성효천발병중적의의，위림상통과익생균적조기간예래규정 Th1／Th2평형제공이론의거。방법ELISA 검측165례소인과민성효천환인（효천조）급동기진행건강체검차합격적150례인동（대조조）적혈청 IFN-γ여 IL-4수평，동시，사용실시형광정량 PCR 법검측분편중쌍기간균적수량。재응용쌍기간균제제조절장도균군6개월후재차검측상술지표，결합림상상관검사자료，대검측결과진행통계학분석。결과효천조초입원시혈장 IFN-γ저우대조조，차이유통계학의의（P ＜0．05），IL-4적수평고우대조조，분편중쌍기간균수량소우대조조，차이유통계학의의（P ＜0．05）。경과6개월적익생균제제적조리，효천조혈장 IFN-γ수평승고，IL-4수평칙하강，분편중쌍기간균적수량증다，여초입원시비교차이균유통계학의의（P ＜0．05）。결론재인동효천환인중，장도균군적평형관계도기면역시부평형，량자상호영향，림상응조기진행간예조절장도균군화면역평형。
Objective To explore the pathogenesis and clinical value of Th1/Th2 and normal intestinal flora from the angle of immunological and intestinal micro environment balance by detecting the plasma interferon-γ(IFN-γ)and interleukin-4(IL-4)and fecal bifidobacteria in Children with Allergic Asthma,so as to provide a theoretical basis through early intervention of probiotics to correct the Th1/Th2 balance.Methods The levels of blood plasma IFN-γ,IL-4 and fecal bifidobacteria amount of 165 cases of chil-dren with allergic asthma and 150 health students as control group were detected by ELISA and real time fluorescence PCR at the same time.Retesting these above indexes after applying bifidobacteria regulation of intestinal flora for 6 months.The clinical relative factors and experimental data were statistically analyzed.Results The level of IFN-γof asthmatic children were remarkably lower than those in the control group(P <0.05),while the level of IL-4 of asthmatic children were remarkably higher than those in the control group(P <0.05),and the amount of bifidobacteria of asthmatic children were remarkably lower than those in the control group(P <0.05).The level of IFN-γand bifidobacteria of asthmatic children improved remarkably and the level of IL-4 decreased significantly after applying bifidobacteria regulation of intestinal flora for 6 months.All of them had remarkable statistically signifi-cant.Conclusion The immune balance was affected by the intestinal flora balance of vice versa.This would provide an important theo-retical support to prevent the occurrence of allergic asthma by the early probiotics regulation of intestinal flora and immune balance.