CAJ | 학술논문

目的：探讨β-内酰胺类抗菌药物对婴儿肠道菌群的影响。方法选取0～1岁婴儿，应用16S rRNA 荧光定量 PCR技术，分别测定β-内酰胺类抗菌药物使用前，使用第3 d、5天时，及治愈后第7天粪便中双歧杆菌、乳酸杆菌、肠球菌、大肠杆菌的水平。结果使用过β-内酰胺类抗菌药物者粪便中双歧杆菌、乳酸杆菌、大肠杆菌和肠球菌的数量与未使用者比较，差异无统计学意义（P ＞0．05）。粪便双歧杆菌、乳酸杆菌含量随治疗时间的延长而增加，差异有统计学意义（P ＜0．05）。未使用抗菌药物的患儿肠道双歧杆菌和乳酸杆菌的恢复明显快于使用过抗菌药物的患儿，差异有统计学意义（P ＜0．05）。结论β-内酰胺类抗菌药物对婴儿肠道菌群有普遍的杀灭作用，对婴儿肠道菌群改变的影响是轻微的。β-内酰胺类抗菌药物的使用会延迟患儿肠道微生态的恢复，但若合理使用对疾病的治疗有积极作用。
목적：탐토β-내선알류항균약물대영인장도균군적영향。방법선취0～1세영인，응용16S rRNA 형광정량 PCR기술，분별측정β-내선알류항균약물사용전，사용제3 d、5천시，급치유후제7천분편중쌍기간균、유산간균、장구균、대장간균적수평。결과사용과β-내선알류항균약물자분편중쌍기간균、유산간균、대장간균화장구균적수량여미사용자비교，차이무통계학의의（P ＞0．05）。분편쌍기간균、유산간균함량수치료시간적연장이증가，차이유통계학의의（P ＜0．05）。미사용항균약물적환인장도쌍기간균화유산간균적회복명현쾌우사용과항균약물적환인，차이유통계학의의（P ＜0．05）。결론β-내선알류항균약물대영인장도균군유보편적살멸작용，대영인장도균군개변적영향시경미적。β-내선알류항균약물적사용회연지환인장도미생태적회복，단약합리사용대질병적치료유적겁작용。
Objective To investigate the influence of β-lactam antimicrobial drugs on infants′intestinal flora.Methods Infants from 0 to 1 years old were enrolled in the study,who′s feces samples were tested for Bacillus bifidus,lactobacillus,enterococcus and E.coli by using 16S rRNA quantitative PCR technique before using antibiotics,on the third and fifth day duringβ-lactam antibiotics treatment and the seventh day after treatment.Results The numbers of Bacillus bifidus,lactobacillus,E.coli and enterococci detec-ted in usingβ-lactam antibiotics group were not statistically different from those in not using antibiotics group(P >0.05).The num-bers of Bacillus bifidus and lactobacillus increased with the treatment process,the differencies was statistically significant(P <0.05).The recovery of the numbers of intestinal Bacillus bifidus and lactobacillus in not using antibiotics group was significantly faster than using antibiotics group,the difference was statistically significant(P <0.05).Conclusion β-lactam antibiotics have gen-eral killing effect on the intestinal flora in infants,which could recover to normal.The recovery of intestinal flora could be delayed if antibiotics are used,however,reasonable antibiotics treatment would be very helpful in the treatment of primary diseases.