CAJ | 학술논문

目的：观察丹参酮对心肌梗死家兔模型恶性心律失常的作用，并探讨丹参酮导致的钙调蛋白（CaM）信号传导系统改变与恶性心律失常发生率降低之间的关系。方法选取健康大耳家兔共90只，随机分为3组：心肌梗死模型家兔（无干预组）、心肌梗死丹参酮干预家兔（干预组）和假手术对照家兔（对照组），每组30只。对3组家兔恶性心律失常发生率、CaM、钙调蛋白激酶Ⅱ（CaMK-Ⅱ）活性、跨室壁复极离散度（TDR）、心肌细胞内 Ca2＋浓度进行统计并比较。结果对照组未发生恶性心肌梗死，无干预组和干预组恶性心律失常发生率均高于对照组，差异有统计学意义（P ＜0．05）；无干预组恶性心律失常率（60．0％）高于干预组（10．0％），差异有统计学意义（P ＜0．05）。无干预组和干预组家兔的 CaM 蛋白水平分别是对照组家兔的1．483倍和1．321倍，差异均有统计学意义（P ＜0．05）；无干预组家兔 CaM 蛋白水平高于干预组，差异有统计学意义（P ＜0．05）。3组间 CaMKⅡ的活性比较差异无统计学意义（P ＞0．05）。无干预组血清钙离子水平高于对照组和干预组，差异有统计学意义（P ＜0．05）；干预组低于无干预组，差异有统计学意义（P ＜0．05）。干预组 TDR 低于无干预组，这两组 TDR 均高于对照组，差异有统计学意义（P ＜0．05）。干预组梗死心肌单细胞水平胞内钙浓度低于无干预组，差异有统计学意义（P ＜0．05）。结论CaM 信号传导系统的相关信号因子的水平对心肌梗死后恶性心律失常有提示作用。丹参酮可以通过抑制 CaM 信号传导系统而在预防心肌梗死后恶性心律失常中发挥重要作用。
목적：관찰단삼동대심기경사가토모형악성심률실상적작용，병탐토단삼동도치적개조단백（CaM）신호전도계통개변여악성심률실상발생솔강저지간적관계。방법선취건강대이가토공90지，수궤분위3조：심기경사모형가토（무간예조）、심기경사단삼동간예가토（간예조）화가수술대조가토（대조조），매조30지。대3조가토악성심률실상발생솔、CaM、개조단백격매Ⅱ（CaMK-Ⅱ）활성、과실벽복겁리산도（TDR）、심기세포내 Ca2＋농도진행통계병비교。결과대조조미발생악성심기경사，무간예조화간예조악성심률실상발생솔균고우대조조，차이유통계학의의（P ＜0．05）；무간예조악성심률실상솔（60．0％）고우간예조（10．0％），차이유통계학의의（P ＜0．05）。무간예조화간예조가토적 CaM 단백수평분별시대조조가토적1．483배화1．321배，차이균유통계학의의（P ＜0．05）；무간예조가토 CaM 단백수평고우간예조，차이유통계학의의（P ＜0．05）。3조간 CaMKⅡ적활성비교차이무통계학의의（P ＞0．05）。무간예조혈청개리자수평고우대조조화간예조，차이유통계학의의（P ＜0．05）；간예조저우무간예조，차이유통계학의의（P ＜0．05）。간예조 TDR 저 우 무 간예조，저량조 TDR 균고우대조조，차 이 유 통 계 학의의（P ＜0．05）。간예조경사심기단세포수평포내개농도저우무간예조，차이유통계학의의（P ＜0．05）。결론CaM 신호전도계통적상관신호인자적수평대심기경사후악성심률실상유제시작용。단삼동가이통과억제 CaM 신호전도계통이재예방심기경사후악성심률실상중발휘중요작용。
Objective To observe the effects of tanshinone on malignant arrhythmias which occured in rabbit model of myocardi-al infarction,and explore the relationship between tanshinone induced calmodulin(CaM)signal system changes and the incidence of malignant arrhythmias after myocardial infarction.Methods A total of 90 healthy big ear rabbits were selected and randomly divid-ed into 3 groups:myocardial infarction model rabbits(non-intervention group)and myocardial infarction model rabbits with tanshi-none intervention(intervention group)and sham operated rabbits(control group),30 rats in each group.The incidence of malignant arrhythmias,calmodulin(CaM),calmodulin kinaseⅡ(CaMKⅡ),transmural dispersity of repolarization(TDR)and the concentra-tion of Ca2 + in myocardial cells were statistically analysed and compared among the 3 groups.Results The control group did not undergo malignant myocardial infarction,in non-intervention group and intervention group,malignant arrhythmia incidence was higher than that in control group(P <0.05);in non-intervention group,malignant arrhythmia rate(60.00%)was higher than that in intervention group(10%)(P <0.05 ).The CaM concentration in non-intervention and intervention group rabbits were 1.483 and 1.321 times of control group rabbits respectively,the difference was statistically significant(P <0.05);CaM protein levels of non-intervention group was higher than that of intervention group(P <0.05).No statistical significance of CaMKⅡ activity among the 3 groups were observed(P >0.05).Serum Ca2 + concentration in non-intervention group was higher than that in control group and intervention group(P <0.05);serum Ca2 + concentration in control group was lower than that in intervention group(P <0.05).The TDR of intervention group is lower than that of non-intervention group,the two groups′TDR were both higher than that of control group(P <0.05).At single cell level,the intracellular calcium concentration of intervention group was lower than that of non-inter-vention group (P <0.05).Conclusion The relevant signal factors of CaM signal transduction system have implications on malig-nant arrhythmia after myocardial infarction.Tanshinone can inhibit CaM signaling system and play an important role in the preven-tion of malignant arrhythmia after myocardial infarction.