组织工程与重建外科杂志
組織工程與重建外科雜誌
조직공정여중건외과잡지
JOURNAL OF TISSUE ENGINEERING AND RECONSTRUCTIVE SURGERY
2015年
1期
19-22,25
,共5页
颅缝细胞%碱性成纤维生长因子%骨形成蛋白%成骨分化
顱縫細胞%堿性成纖維生長因子%骨形成蛋白%成骨分化
로봉세포%감성성섬유생장인자%골형성단백%성골분화
Calvarial suture cell%Basic fibroblast growth factor%Bone morphogenetic protein%Osteoblastic differentiation
目的:探讨碱性成纤维生长因子2(FGF-2)与骨形成蛋白2(BMP-2)在颅缝细胞成骨分化中的相互作用及其机制。方法获取新生SD大鼠颅骨矢状缝及冠状缝处颅缝细胞,在培养体系中添加FGF-2,观察BMP-2表达情况。同时在培养体系中添加FGF-2及BMP-2,ALP染色、矿化染色、qPCR检测成骨标志物,观察颅缝细胞成骨分化情况。添加BMP-2抑制剂Noggin后,观察颅缝细胞成骨分化的转归。结果 FGF-2可促进BMP-2在颅缝细胞中的表达,呈浓度依赖性及时间依赖性;两者同时作用颅缝细胞可促进其晚期成骨分化,抑制其早期成骨分化。 Noggin阻断BMP-2信号通道后,FGF-2及FGF-2+BMP-2促进颅缝细胞晚期成骨分化作用均减弱。结论 BMP-2是FGF-2调控颅缝细胞晚期成骨分化不可或缺的下游因子。
目的:探討堿性成纖維生長因子2(FGF-2)與骨形成蛋白2(BMP-2)在顱縫細胞成骨分化中的相互作用及其機製。方法穫取新生SD大鼠顱骨矢狀縫及冠狀縫處顱縫細胞,在培養體繫中添加FGF-2,觀察BMP-2錶達情況。同時在培養體繫中添加FGF-2及BMP-2,ALP染色、礦化染色、qPCR檢測成骨標誌物,觀察顱縫細胞成骨分化情況。添加BMP-2抑製劑Noggin後,觀察顱縫細胞成骨分化的轉歸。結果 FGF-2可促進BMP-2在顱縫細胞中的錶達,呈濃度依賴性及時間依賴性;兩者同時作用顱縫細胞可促進其晚期成骨分化,抑製其早期成骨分化。 Noggin阻斷BMP-2信號通道後,FGF-2及FGF-2+BMP-2促進顱縫細胞晚期成骨分化作用均減弱。結論 BMP-2是FGF-2調控顱縫細胞晚期成骨分化不可或缺的下遊因子。
목적:탐토감성성섬유생장인자2(FGF-2)여골형성단백2(BMP-2)재로봉세포성골분화중적상호작용급기궤제。방법획취신생SD대서로골시상봉급관상봉처로봉세포,재배양체계중첨가FGF-2,관찰BMP-2표체정황。동시재배양체계중첨가FGF-2급BMP-2,ALP염색、광화염색、qPCR검측성골표지물,관찰로봉세포성골분화정황。첨가BMP-2억제제Noggin후,관찰로봉세포성골분화적전귀。결과 FGF-2가촉진BMP-2재로봉세포중적표체,정농도의뢰성급시간의뢰성;량자동시작용로봉세포가촉진기만기성골분화,억제기조기성골분화。 Noggin조단BMP-2신호통도후,FGF-2급FGF-2+BMP-2촉진로봉세포만기성골분화작용균감약。결론 BMP-2시FGF-2조공로봉세포만기성골분화불가혹결적하유인자。
Objective To explore the interaction of FGF-2 and BMP-2 in osteoblastic differentiation of calvarial suture cells. Methods Neonatal calvarial suture cells of SD rat were harvested. FGF-2 was added into cell cultures and BMP-2 expression in cranial suture cells was observed. Meanwhile, FGF-2 and BMP-2 were both added into cell cultures and the osteoblastic differentiation of cranial suture cells was observed by ALP staining, mineralized nodule staining and qPCR. Then Noggin was added to observe the changes of cells’ osteoblastic differentiation. Results BMP-2 expression increased in a time-dependent manner after the cells treated with FGF-2 and increased in a dose-dependent manner up to 50 ng/ml FGF-2, after which BMP-2 expression reached a plateau;After FGF-2 and BMP-2 co-stimulation, the expression of early marker of osteoblast differentiation (COL-1) was decreased while the expression of late markers (ALP, OC and BSP) were increased to accelerate mineralization. The natural BMP antagonist Noggin inhibited the expression of FGF2-induced OC and BSP by 1.40-fold and 1.41-fold respectively, and inhibited the expression of FGF2- and BMP2-induced OC and BSP by 1.26-fold and 1.20-fold respectively. Conclusion BMP2 is a downstream target of FGF-2, and BMP-2 signals are required for FGF-2-dependent induction of later-stage osteoblast differentiation in cranial suture cells.