CAJ | 학술논문

目的：探讨缺血后处理对梗阻性黄疸肾缺血再灌注损伤大鼠血清TNF-α水平的影响。方法60只SD大鼠随机分成梗阻性黄疸对照组（S组）、梗阻性黄疸肾缺血再灌注组（I／R组）、梗阻性黄疸肾缺血再灌注缺血后处理组（IPO组），每组20只。根据缺血后再灌注时间点各组又分为再灌注0 h（T0）、1 h（T1）、3 h（T2）、6 h（T3）4个亚组各5例。检测再灌注后的各时点血清中尿素氮（BUN）、肌酐（Cr）、TNF-α水平，观察肾组织的病理改变。结果 I／R组和IPO组血清BUN、Cr、TNF-α水平均高于S组（P均＜0．05）， IPO组T2时间点血清Cr、BUN、TNF-α水平均低于I／R组（ P均＜0．05）。结论缺血后处理可以下调大鼠血清TNF-α水平，从而减轻梗阻性黄疸大鼠肾缺血再灌注损伤，其机制可能与抑制炎症反应有关。
목적：탐토결혈후처리대경조성황달신결혈재관주손상대서혈청TNF-α수평적영향。방법60지SD대서수궤분성경조성황달대조조（S조）、경조성황달신결혈재관주조（I／R조）、경조성황달신결혈재관주결혈후처리조（IPO조），매조20지。근거결혈후재관주시간점각조우분위재관주0 h（T0）、1 h（T1）、3 h（T2）、6 h（T3）4개아조각5례。검측재관주후적각시점혈청중뇨소담（BUN）、기항（Cr）、TNF-α수평，관찰신조직적병리개변。결과 I／R조화IPO조혈청BUN、Cr、TNF-α수평균고우S조（P균＜0．05）， IPO조T2시간점혈청Cr、BUN、TNF-α수평균저우I／R조（ P균＜0．05）。결론결혈후처리가이하조대서혈청TNF-α수평，종이감경경조성황달대서신결혈재관주손상，기궤제가능여억제염증반응유관。
Objective To investigate effect of ischemic postconditioning on kidney ischemia-reperfusion injury and the expression of TNF-αin obstructive jaundice rats .Methods Totally 60 SD rats were randomly divided into three groups ( n=20):obstructive jaundice group (group S), obstructive jaundice renal Ischemia-reperfusion group( group I/R), ob-structive jaundice +ischemic postconditioning group ( group IPO ) .According to the time of ischemia reperfusion , each group was divided into four subgroups (n=5):0 h reperfusion (T0), 1 h reperfusion (T1), 3 h reperfusion (T2), 6 h reperfusion (T3) .In each period, the serum urea of nitrogen (BUN), creatinine (Cr) and TNF-αwere collected and ob-served, kidney pathological changes were observed .Results Compared with group S, the serum levels of BUN and Cr in-creased, TNF-αin renal tissue were upregulated in groups I/R and IPO (all P<0.05).At T2, compared with group I/R, the serum levels of BUN, Cr and TNF-αdecreased (all P<0.05).Conclusions Ischemic postconditioning can downreg-ulate the expression of TNF-αand alleviate the renal ischemia-reperfusion injury in obstructive jaundice rats , whith may be through inhibiting inflammatory reactions .