CAJ | 학술논문

目的：评估槲皮素对缺血再灌注大鼠肝脏损伤大鼠的保护作用。方法健康大鼠80只被随机分为对照组5只、假手术组25只、缺血再灌注组25只和槲皮素组25只，后3组再按不同灌注时限（0、6、12、24、72 h）分为5个亚组，每个亚组5只。分别于实验结束后留取大鼠血液及肝脏组织，用全自动生化分析仪检测血清中 ALT、AST水平，竞争抑制ELISA法检测血清中IL-1β、IL-6、IL-8、TNF-α水平；HE染色法观察肝脏的形态学改变，并用原位末端标记法检测肝脏细胞的凋亡情况。结果肝脏缺血再灌注组各时点血清中ALT、AST、IL-1β、IL-6、IL-8、TNF-α均有所升高，且以12 h最高，与对照组、假手术组相比差异有统计学意义（P均＜0．05）；除IL-6外，槲皮素组各时点以上检测指标与缺血再灌注组比较有统计学差异（P均＜0．05）。光镜下，对照组、假手术组肝脏无明显改变；而在缺血再灌注后12 h，肝细胞变性明显，肝索结构消失，部分肝细胞灶状坏死；槲皮素组的肝脏仅部分细胞变性，部分肝索结构存在，该形态学变化与细胞凋亡指数的检测结果一致。结论槲皮素能够有效地改善大鼠肝脏缺血再灌注损伤。
목적：평고곡피소대결혈재관주대서간장손상대서적보호작용。방법건강대서80지피수궤분위대조조5지、가수술조25지、결혈재관주조25지화곡피소조25지，후3조재안불동관주시한（0、6、12、24、72 h）분위5개아조，매개아조5지。분별우실험결속후류취대서혈액급간장조직，용전자동생화분석의검측혈청중 ALT、AST수평，경쟁억제ELISA법검측혈청중IL-1β、IL-6、IL-8、TNF-α수평；HE염색법관찰간장적형태학개변，병용원위말단표기법검측간장세포적조망정황。결과간장결혈재관주조각시점혈청중ALT、AST、IL-1β、IL-6、IL-8、TNF-α균유소승고，차이12 h최고，여대조조、가수술조상비차이유통계학의의（P균＜0．05）；제IL-6외，곡피소조각시점이상검측지표여결혈재관주조비교유통계학차이（P균＜0．05）。광경하，대조조、가수술조간장무명현개변；이재결혈재관주후12 h，간세포변성명현，간색결구소실，부분간세포조상배사；곡피소조적간장부부분세포변성，부분간색결구존재，해형태학변화여세포조망지수적검측결과일치。결론곡피소능구유효지개선대서간장결혈재관주손상。
Objective To establish rat ischemia reperfusion injury model and evaluate the protection effect of querce -tin against liver ischemia reperfusion injury in rats .Methods A total of 80 healthy rats were randomly divided into control group(n=5), sham group(n=25), ischemia reperfusion injury group(n=25) and quercetin group(n=25).The later three groups were divided into 0, 6, 12, 24, 72 h subgroups(n=5), respectively.At the end of the experiment, the blood samples and liver tissues were collected .The levels of ALT, AST, IL-1β, IL-6, IL-8 and TNF-αwere determined by automatic biochemistry analyzer and competitive inhibition ELISA , respectively .The morphology change of liver were de-tected by HE staining, and the apoptosis of liver cells were detected by TUNEL .Results In 0, 6, 12, 24, 72 h sub-group, the levels of ALT, AST, IL-1β, IL-6, IL-8 and TNF-αincreased, especially in 12 h group, which had significant statistically difference compared with control or sham group (all P<0.05).Under light microscope, there was no signifi-cant change in control or sham group .In ischemia reperfusion injury group , liver cells degenerated significantly , liver cord structure disappeared, some liver cells necrosed.However, in quercetin group, only part liver cells degenerated , part liver cord structure exsited , which was consistent with the results of apoptosis index .Conclusion Quercetin is beneficial for liver ischemia reperfusion injury .