山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2015年
5期
16-18
,共3页
王冰%屈朋欢%王艳华%崔乃鹏%蔡建辉%陈保平
王冰%屈朋歡%王豔華%崔迺鵬%蔡建輝%陳保平
왕빙%굴붕환%왕염화%최내붕%채건휘%진보평
B16 F10黑色素瘤%全身放射线照射%过继性免疫治疗%小鼠
B16 F10黑色素瘤%全身放射線照射%過繼性免疫治療%小鼠
B16 F10흑색소류%전신방사선조사%과계성면역치료%소서
B16F10 melanoma%total body irradiation%adoptive immunotherapy%mice
目的:观察全身放射线照射( TBI)对B16F10黑色素瘤小鼠移植肿瘤生长及小鼠存活的影响。方法建立C57BL/6小鼠B16F10黑色素瘤移植肿瘤模型,采用不同剂量分别对小鼠进行TBI,观察小鼠移植肿瘤的生长和小鼠的存活情况;检测放疗后小鼠外周血白细胞水平。结果不同剂量TBI对各组小鼠肿瘤面积及存活率无影响(P均>0.05)。给予7 Gy TBI 10 d后,B16F10荷瘤小鼠外周血白细胞水平下降(P<0.05)。结论 TBI不影响B16 F10黑色素瘤小鼠移植肿瘤生长及荷瘤小鼠的生存;7 Gy TBI可改变荷瘤小鼠外周血白细胞水平,有利于肿瘤免疫治疗。
目的:觀察全身放射線照射( TBI)對B16F10黑色素瘤小鼠移植腫瘤生長及小鼠存活的影響。方法建立C57BL/6小鼠B16F10黑色素瘤移植腫瘤模型,採用不同劑量分彆對小鼠進行TBI,觀察小鼠移植腫瘤的生長和小鼠的存活情況;檢測放療後小鼠外週血白細胞水平。結果不同劑量TBI對各組小鼠腫瘤麵積及存活率無影響(P均>0.05)。給予7 Gy TBI 10 d後,B16F10荷瘤小鼠外週血白細胞水平下降(P<0.05)。結論 TBI不影響B16 F10黑色素瘤小鼠移植腫瘤生長及荷瘤小鼠的生存;7 Gy TBI可改變荷瘤小鼠外週血白細胞水平,有利于腫瘤免疫治療。
목적:관찰전신방사선조사( TBI)대B16F10흑색소류소서이식종류생장급소서존활적영향。방법건립C57BL/6소서B16F10흑색소류이식종류모형,채용불동제량분별대소서진행TBI,관찰소서이식종류적생장화소서적존활정황;검측방료후소서외주혈백세포수평。결과불동제량TBI대각조소서종류면적급존활솔무영향(P균>0.05)。급여7 Gy TBI 10 d후,B16F10하류소서외주혈백세포수평하강(P<0.05)。결론 TBI불영향B16 F10흑색소류소서이식종류생장급하류소서적생존;7 Gy TBI가개변하류소서외주혈백세포수평,유리우종류면역치료。
Objective To investigate effects of total body irradiation (TBI) on tumor growth and B16F10 melanoma-bearing mice survival.Methods C57BL/6 mice bearing B16-melanoma tumors were irradiated with 0, 5, or 7 Gy total body irradiation ( TBI) , or 7 Gy TBI pus bone marrow transplantation .Tumor areas were measured every 3 days to assess the influences of irradiation treatment on tumor regression .B16-melanoma bearing mice were irradiated with 7 Gy TBI and peripheral blood were harvested at days 1, 3, 5, 7, 9, 11 and 13 after irradiation to test WBC levels .Results TBI with variant dosage on the B 16-melanoma-bearing mice did not influence tumor regression compared with control group ( all P>0.05).WBC levels significantly decreased in the B16F10 melanoma-bearing mice on 10 d after 7 Gy TBI(P<0.05). Conclusion TBI dose do not influence tumor growth and survival of B 16F10 melanoma-bearing mice.Seven Gy TBI can alter WBC levels of peripheral bloods in B 16F10 melanoma-bearing mice, which helps to tumor immunotherapy .