CAJ | 학술논문

目的：分析细胞色素P4501A1（CYP1A1）和谷胱甘肽转移酶P1（GSTP1）基因多态性与抗结核药物性肝损伤（ ADLI ）的相关性。方法抗结核治疗致肝损伤的结核病患者127例为病例组，127例无肝损伤者为对照组，收集其环境因素暴露资料及静脉血。 CYP1A1基因Msp I位点和GSTP1基因 Ile105 Val位点多态性检测用聚合酶链反应限制性片段长度多态法（ PCR－RFLP ）。用SPSS 17．0软件对环境因素和基因型进行单因素和多因素条件Logistic 回归分析。结果 CYP1A1基因Msp I位点各基因型在2组间的分布差异无统计学意义；GSTP1基因Ile105Val位点AA、AG、GG基因型在病例组和对照组频率分别为55．91％，32．28％，11．81％和70．08％，26．77％，3．15％，GG基因型在2组分布差异有统计学意义（ P＜0．05）。 GG基因型的危险度比值比（ OR）值为4．701。单因素条件Logistic回归分析发现，饮酒为ADLI的危险因素；多因素分析中调整饮酒因素的影响后，GSTP1基因 Ile105 Val位点GG基因型仍为ADLI的危险基因型。结论 GSTP1基因Ile105 Val位点多态性与ADLI的发生有关，携带GG基因型的个体是危险人群。
목적：분석세포색소P4501A1（CYP1A1）화곡광감태전이매P1（GSTP1）기인다태성여항결핵약물성간손상（ ADLI ）적상관성。방법항결핵치료치간손상적결핵병환자127례위병례조，127례무간손상자위대조조，수집기배경인소폭로자료급정맥혈。 CYP1A1기인Msp I위점화GSTP1기인 Ile105 Val위점다태성검측용취합매련반응한제성편단장도다태법（ PCR－RFLP ）。용SPSS 17．0연건대배경인소화기인형진행단인소화다인소조건Logistic 회귀분석。결과 CYP1A1기인Msp I위점각기인형재2조간적분포차이무통계학의의；GSTP1기인Ile105Val위점AA、AG、GG기인형재병례조화대조조빈솔분별위55．91％，32．28％，11．81％화70．08％，26．77％，3．15％，GG기인형재2조분포차이유통계학의의（ P＜0．05）。 GG기인형적위험도비치비（ OR）치위4．701。단인소조건Logistic회귀분석발현，음주위ADLI적위험인소；다인소분석중조정음주인소적영향후，GSTP1기인 Ile105 Val위점GG기인형잉위ADLI적위험기인형。결론 GSTP1기인Ile105 Val위점다태성여ADLI적발생유관，휴대GG기인형적개체시위험인군。
Objective To retrospectively investigate the relationship be-tween the gene polymorphisms of cytochrome P450 1 A1 \glutathione transferase P1 ( CYP1 A1 or GSTP1 ) and anti -tuberculosis drug -induced liver injury ( ADLI).Methods Total 127 cases and 127 con-trols were collected in 1∶1 match.Their exposure data of data and blood samples were collected. The genotypes were detected by polymerase chain reaction and restriction fragment length polymorphism technique ( PCR -RFLP ) method SPSS 17.0 software was used to do Logistic regression analysis on environmental factors and genotype univariate and multivariate analysis.Results The allele frequencies of gene CYP1 A1 genes in two groups had no statistical difference.While the frequencies of AA, AG and GG genotypes at Ile105 Val site of GSTP1 gene were 55.91%, 32.28%, 11.81% in case group and 70.08%, 26.77%, 3.15%in control group.The analysis demonstrated that the frequencies of GSTP1 gene Ile105 Val genotypes in case group were significantly higher than those in control group ( P<0.05 ) .Univariate Logistic re-gression analysis showed that alcohol was the risk factor of ADLI, and after the regulation of certain factor in multivariate analysis, the GG genotype at Ile105 Val site of GSTP1 gene was still the risk factor of ADLI.Conclusion The polymorphim of Ile105 Val site of GSTP1 gene is correlated with the occur-rence of ADLI and GG carriers are considered as risk population.