CAJ | 학술논문

目的：评价银花泌炎灵体外抗菌活性及与临床常用抗菌药物的体外联合作用。方法对来自全国18家医院近3年临床分离的285株菌进行了最低抑菌浓度（ MIC）测定， MIC测定用琼脂二倍稀释法，联合药敏测定采用棋盘法。结果银花泌炎灵对葡萄球菌和奇异变形杆菌具有较好的抗菌活性，MIC值在3．13～6．25 mg? mL－1。不论细菌是否产超广谱β内酰胺酶（ ESBLs）或是否为甲氧西林耐药株或是否为环丙沙星耐药株，银花泌炎灵均表现出同样的抗菌作用。联合药敏结果显示，银花泌炎灵与阿莫西林／克拉维酸、头孢呋辛以及左氧氟沙星联合对革兰阳性菌，特别是甲氧西林耐药金黄色葡萄球菌（ MRSA ）有较好的协同作用。银花泌炎灵与头孢呋辛联合对粪肠球菌有协同作用，与阿莫西林／克拉维酸联合对大肠埃希菌表现出100％的相加作用。结论银花泌炎灵与β内酰胺类和喹诺酮类联合，对革兰阳性菌表现出一定的协同作用，对革兰阴性菌表现出一定的相加作用。
목적：평개은화비염령체외항균활성급여림상상용항균약물적체외연합작용。방법대래자전국18가의원근3년림상분리적285주균진행료최저억균농도（ MIC）측정， MIC측정용경지이배희석법，연합약민측정채용기반법。결과은화비염령대포도구균화기이변형간균구유교호적항균활성，MIC치재3．13～6．25 mg? mL－1。불론세균시부산초엄보β내선알매（ ESBLs）혹시부위갑양서림내약주혹시부위배병사성내약주，은화비염령균표현출동양적항균작용。연합약민결과현시，은화비염령여아막서림／극랍유산、두포부신이급좌양불사성연합대혁란양성균，특별시갑양서림내약금황색포도구균（ MRSA ）유교호적협동작용。은화비염령여두포부신연합대분장구균유협동작용，여아막서림／극랍유산연합대대장애희균표현출100％적상가작용。결론은화비염령여β내선알류화규낙동류연합，대혁란양성균표현출일정적협동작용，대혁란음성균표현출일정적상가작용。
Objective To evaluation in vitro activity and synergy of Yin-hua Miyan Ling against main clinical isolated strains.Methods All of 285 stains from 18 tertiary hospitals in recent three years were studied. Minimal inhibition concentrations ( MIC ) were tested by Agar dilution method and combination effects were measured by Checkerboard method. Results Yinhua Miyan Ling showed more in vitro activity against Sta-phylococcus and Proteus mirabilis, MIC values from 3.13~6.25 mg? mL-1 . On the other hand, Yinhua Miyan Ling showed the same antibiotic activi-ty against extended-spectrumβlactamases ( ESBLs) positive and nega-tive strains or methecillin susceptible and resistant Staphylococcus.Syner-gy was detected with Yinhua Miyan Ling in combination with amoxillin/clavulanate, cefuroxime and levofloxacin against gram-positive strains, especially methicillin-resistant Staphylococcus aureus ( MRSA).Against E.faecalis, synergy was observed in Yinhua Miyan Ling and cefuroxime. Additive effect was found between Yinhua Miyan Ling and amoxillin/clavulanate against 100%E.coli.Conclusion Synergy was found with Yinhua Miyan Ling in combination withβlactams and quinolones against gram -positive strains and additive effect was found against gram-negative bacilli.