CAJ | 학술논문

目的：探讨磷霉素（ FOS）对鲍曼不动杆菌生物膜的破坏作用以及与左氧氟沙星（ LFX）的联合杀菌效果。方法选取临床分离鲍曼不动杆菌菌株构建体外生物膜模型，微量肉汤稀释法测定FOS及LFX的最低抑菌浓度（ MIC ），连续稀释法测定生物膜内活菌计数，结晶紫染色法半定量生物膜。用单因素方差分析进行统计处理。结果生物膜经抗生素作用24 h后，生物膜半定量显示，FOS组及FOS＋LFX组吸光度值均少于空白对照组，差异有统计学意义（ P＜0．01）；但LFX组吸光度值与空白对照组比较，差异无统计学意义。 FOS组及LFX组的生物膜内活菌计数与空白对照组比较，差异无统计学意义；FOS＋LFX组第4，8，24 h膜内活菌计数均少于空白对照组（ P＜0．01）。结论 FOS能破坏鲍曼不动杆菌已形成的生物膜，并可增强LFX对生物膜内鲍曼不动杆菌的清除作用。
목적：탐토린매소（ FOS）대포만불동간균생물막적파배작용이급여좌양불사성（ LFX）적연합살균효과。방법선취림상분리포만불동간균균주구건체외생물막모형，미량육탕희석법측정FOS급LFX적최저억균농도（ MIC ），련속희석법측정생물막내활균계수，결정자염색법반정량생물막。용단인소방차분석진행통계처리。결과생물막경항생소작용24 h후，생물막반정량현시，FOS조급FOS＋LFX조흡광도치균소우공백대조조，차이유통계학의의（ P＜0．01）；단LFX조흡광도치여공백대조조비교，차이무통계학의의。 FOS조급LFX조적생물막내활균계수여공백대조조비교，차이무통계학의의；FOS＋LFX조제4，8，24 h막내활균계수균소우공백대조조（ P＜0．01）。결론 FOS능파배포만불동간균이형성적생물막，병가증강LFX대생물막내포만불동간균적청제작용。
Objective To observe the in vitro destructive effect of fos-fomycin ( FOS ) on the biofilm of Acinetobacter baumannii and the synergistic antibacterial activity in combination with levofloxacin ( LFX) .Methods The biofilm model was established by clinical isolates of Acinetobacter baumannii -48876 as the study strain.The minimum inhibitory concentrations ( MIC ) of FOS and LFX were measured by doubling dilution. The viable count of biofilm carrier were determined by continuous dilution method and the biofilm quanti-tation by crystal violet staining method.Data were analyzed by single factor analysis of variance ( One-Way ANVOA).Results When the biofilm being reacted with antibiotic for 24 hours, the quantitation of biofilm showed the absorbances of FOS group and FOS plus LFX group were significantly less than that of the control group ( P<0.01 ) .While there was no statistical difference found in absor-bance value in LFX group and the control group.At the 4 th , 8 th and 24 th hour, the number of bacteria in biofilm of FOS plus LFX group was significantly less than that in the control group ( P<0.01 ) , While there was no statistical difference found between FOS or LFX group and control group.Conclusion FOS can destroy the biofilm formed by Acinetobacter baumannii, and the Acinetobacter baumannii in the biofilm carrier could be significantly reduced while plus LFX.