CAJ | 학술논문

目的：探讨解氏肾癌一号方对小鼠肾癌的抑制作用及对Bcl-2和Bax蛋白表达的影响。方法建立肾癌小鼠动物模型,分为生理盐水对照组、中药对照组、白介素-2组、解氏肾癌一号方组,计算各组抑瘤率以及采用免疫组化法检测凋亡相关蛋白Bcl-2和Bax的表达。结果解氏肾癌一号方组抑瘤率高于生理盐水对照组及白介素-2组,小鼠体重增加。解氏肾癌一号方组小鼠肿瘤组织Bcl-2表达下调,Bax表达上调。结论解氏肾癌一号方可以下调Bcl-2的表达,上调Bax的表达,从而抑制肿瘤生长,改善小鼠的生存质量。
목적：탐토해씨신암일호방대소서신암적억제작용급대Bcl-2화Bax단백표체적영향。방법건립신암소서동물모형,분위생리염수대조조、중약대조조、백개소-2조、해씨신암일호방조,계산각조억류솔이급채용면역조화법검측조망상관단백Bcl-2화Bax적표체。결과해씨신암일호방조억류솔고우생리염수대조조급백개소-2조,소서체중증가。해씨신암일호방조소서종류조직Bcl-2표체하조,Bax표체상조。결론해씨신암일호방가이하조Bcl-2적표체,상조Bax적표체,종이억제종류생장,개선소서적생존질량。
Objective To investigate inhibitory effect and affect on Bcl-2 and Bax protein expression of renal cancer prescription No.1 in mice with renal cancer. Methods The animal models of renal cancer were established and divided into saline control group,Chinese medicine control group,interleukin-2 group and renal cancer prescription NO.1 group. Inhibitory rate of tumor in four groups was calculated and the apoptosis-related protein Bcl-2 and Bax index were de-tected by immuno- histochemistry. Results The inhibitory rate of tumor in renal cancer prescription NO.1 group was higher than that in saline control group and interleukin-2 group respectively,and the weight of mice was increased.The expression of Bcl-2 in renal cancer prescription NO.1 group was lower,but expression of Bax in renal cancer prescrip-tion NO.1 group was higher. Conclusion Renal cancer prescription NO.1 can inhibit the expression of Bcl-2,raise the expression of Bax,and suppress tumor growth,improve the quality of life in mice.