CAJ | 학술논문

目的：探讨鼻咽癌组织和细胞E－钙黏蛋白（ E－cadherin ）基因表达水平与启动子甲基化的相关性，以及患者外周血E－cadherin甲基化作为肿瘤分子标志物的可能。方法采用RT－PCR检测鼻咽癌组织及两种鼻咽癌细胞株HNE1和CNE2中E－cadherin表达水平，以甲基化特异性PCR及硫化测序方法甲基化状态分析鼻咽癌组织、两种鼻咽癌细胞株及鼻咽癌患者外周血中E－cadherin启动子区域的甲基化状态。结果40例鼻咽癌组织中E－cadherin 表达下调率为80％（32／40），甲基化率为65％（26／40），5例正常鼻咽组织中未检测到E－cadherin甲基化，鼻咽癌患者外周血中E－cadherin甲基化率为45％（9／20）。结论基因启动子区域高甲基化状态可能是导致鼻咽癌组织中E－cadherin表达下调的重要机制，经外周血检测E－cadherin甲基化将为鼻咽癌的筛查诊断提供敏感、特异的无创检测方法。
목적：탐토비인암조직화세포E－개점단백（ E－cadherin ）기인표체수평여계동자갑기화적상관성，이급환자외주혈E－cadherin갑기화작위종류분자표지물적가능。방법채용RT－PCR검측비인암조직급량충비인암세포주HNE1화CNE2중E－cadherin표체수평，이갑기화특이성PCR급류화측서방법갑기화상태분석비인암조직、량충비인암세포주급비인암환자외주혈중E－cadherin계동자구역적갑기화상태。결과40례비인암조직중E－cadherin 표체하조솔위80％（32／40），갑기화솔위65％（26／40），5례정상비인조직중미검측도E－cadherin갑기화，비인암환자외주혈중E－cadherin갑기화솔위45％（9／20）。결론기인계동자구역고갑기화상태가능시도치비인암조직중E－cadherin표체하조적중요궤제，경외주혈검측E－cadherin갑기화장위비인암적사사진단제공민감、특이적무창검측방법。
Objective To investigate the expression and methylation status of E -cadherin in nasopharyngeal car-cinoma ( NPC) , and to evaluate efficacy of methylated E -cadherin in peripheral blood as a potential biomarker on the di-agnosis of NPC.Methods RT-PCR and methylation-specific PCR ( MSP) were performed to analyze the mRNA ex-pression levels and methylation status of E -cadherin in primary NPC specimens and two tumor cell lines .MSP was also used to observe the methylated E -cadherin in primary NPC specimens , two tumor cell lines and peripheral blood samples of NPC patients.Results Down-regulated E-cadherin expression was detected in 32 of 40 (80%) primary tumors as well as two NPC cell lines , when compared with normal tissues .Methylated E-cadherin was observed in 26 of 40 (65%) primary tumors but not in normal tissues .Moreover, E-cadherin methylation was also detected in 9 of 20 (45%) periph-eral blood samples of NPC patients .Conclusion Promoter methylation may be one of the mechanisms inducing E -cad-herin silencing .Detection of methylated E -cadherin in peripheral blood suggests potential clinical application for nonin -vasive diagnosis and disease monitoring of NPC .