解放军医药杂志
解放軍醫藥雜誌
해방군의약잡지
MEDICAL&PHARMACEUTICAL JOURNAL OF CHINESE PEOPLE'S LIBERATION ARMY
2015年
2期
85-88
,共4页
牙周疾病%牙髓疾病%牙周源性%微生物%感染
牙週疾病%牙髓疾病%牙週源性%微生物%感染
아주질병%아수질병%아주원성%미생물%감염
Periodontal diseases%Dental Pulp Diseases%Periodontal source%Microorganism%Infection
目的：探讨牙周源性牙周牙髓联合病变微生物感染情况。方法对2013年1—12月就诊的牙周源性牙周牙髓联合病变38例78颗患牙(观察组)和同期正畸拔除的38例78颗牙(对照组),分别采集牙周袋、根管标本,以PCR-DGGE技术检测微生物种类,分析微生物感染情况。结果观察组患牙微生物检出率高于对照组( P<0．05)。观察组牙周袋检出菌属种类为弯曲菌属34颗、放线菌属42颗、梭杆菌属28颗、肠杆菌属19颗和嗜血杆菌属14颗,对照组为奈瑟菌属8颗、放线菌属6颗、弯曲菌属3颗。观察组牙周袋标本菌种条带14.33%~57.85%在同颗牙根管的牙髓标本中存在,但根管牙髓标本中菌种条带有1.32%~67.55%在同颗牙周袋标本中不存在；观察组中菌种条带1.21%~4.38%在对照组同位牙标本中存在,但对照组标本中菌种条带有0~0.57%在观察组同位牙标本中不存在。牙周源性牙周牙髓联合病变与弯曲菌属、放线菌属、梭杆菌属、肠杆菌属、嗜血杆菌属感染有关(r=0.232,P<0．05)。结论牙周源性牙周牙髓联合病变与微生物感染有关,且牙周源性与牙髓源性感染微生物种类既有相似又有不同,临床针对微生物感染种类进行治疗可提高治愈率。
目的：探討牙週源性牙週牙髓聯閤病變微生物感染情況。方法對2013年1—12月就診的牙週源性牙週牙髓聯閤病變38例78顆患牙(觀察組)和同期正畸拔除的38例78顆牙(對照組),分彆採集牙週袋、根管標本,以PCR-DGGE技術檢測微生物種類,分析微生物感染情況。結果觀察組患牙微生物檢齣率高于對照組( P<0．05)。觀察組牙週袋檢齣菌屬種類為彎麯菌屬34顆、放線菌屬42顆、梭桿菌屬28顆、腸桿菌屬19顆和嗜血桿菌屬14顆,對照組為奈瑟菌屬8顆、放線菌屬6顆、彎麯菌屬3顆。觀察組牙週袋標本菌種條帶14.33%~57.85%在同顆牙根管的牙髓標本中存在,但根管牙髓標本中菌種條帶有1.32%~67.55%在同顆牙週袋標本中不存在；觀察組中菌種條帶1.21%~4.38%在對照組同位牙標本中存在,但對照組標本中菌種條帶有0~0.57%在觀察組同位牙標本中不存在。牙週源性牙週牙髓聯閤病變與彎麯菌屬、放線菌屬、梭桿菌屬、腸桿菌屬、嗜血桿菌屬感染有關(r=0.232,P<0．05)。結論牙週源性牙週牙髓聯閤病變與微生物感染有關,且牙週源性與牙髓源性感染微生物種類既有相似又有不同,臨床針對微生物感染種類進行治療可提高治愈率。
목적：탐토아주원성아주아수연합병변미생물감염정황。방법대2013년1—12월취진적아주원성아주아수연합병변38례78과환아(관찰조)화동기정기발제적38례78과아(대조조),분별채집아주대、근관표본,이PCR-DGGE기술검측미생물충류,분석미생물감염정황。결과관찰조환아미생물검출솔고우대조조( P<0．05)。관찰조아주대검출균속충류위만곡균속34과、방선균속42과、사간균속28과、장간균속19과화기혈간균속14과,대조조위내슬균속8과、방선균속6과、만곡균속3과。관찰조아주대표본균충조대14.33%~57.85%재동과아근관적아수표본중존재,단근관아수표본중균충조대유1.32%~67.55%재동과아주대표본중불존재；관찰조중균충조대1.21%~4.38%재대조조동위아표본중존재,단대조조표본중균충조대유0~0.57%재관찰조동위아표본중불존재。아주원성아주아수연합병변여만곡균속、방선균속、사간균속、장간균속、기혈간균속감염유관(r=0.232,P<0．05)。결론아주원성아주아수연합병변여미생물감염유관,차아주원성여아수원성감염미생물충류기유상사우유불동,림상침대미생물감염충류진행치료가제고치유솔。
Objective To explore the microorganism infection in combined periodontal source of periodontal-en-dodontic lesions. Methods A total of 38 patients (78 teeth, observation group) with combined periodontal source of periodontal-endodontic lesions and 38 orthodontic patients (78 teeth, control group) from January to December 2013 were recruited in this study. The samples of periodontal pocket and root canal were detected by PCR-DGGE ( Polymerase Chain Reaction-denaturing gradient gel electrophoresis) for microorganism species, and the microorganism infection was also an-alyzed. Results The isolation rate of microorganism in observation group was significantly higher than that in control group (P<0. 05). There were Campylobacter (34 teeth), Actinomyces (42 teeth), Fusobacterium (28 teeth), Enter-obacter (19 teeth) and Haemophilus (14 teeth) in the observation group, while Neisseria (8 teeth), Actinomyces (6 teeth) and Campylobacter (3 teeth) in control group. In observation group, 14. 33%-57. 85% of strain bands of perio-dontal pocket were same with those of root canal in the same tooth, but 1. 32%-67. 55% of strain bands of root canal did not exist in periodontal pocket in the same tooth; 1. 21%-4. 38% of strain bands of periodontal pocket in observation group existed in the same position tooth of root canal in control group, but 0-0. 57% of strain bands in control group did not exist in the same position tooth of root canal in observation group. The combined periodontal source of periodontal-en-dodontic lesions and infection were closely related with Campylobacter, Actinomyces, Fusobacterium, Enterobacter and Haemophilus (r=0. 232, P<0. 05). Conclusion The combined periodontal source of periodontal-endodontic lesions is closely related with the microorganism infection, and periodontal source and dental pulp source of periodontal-endodontic lesions are partly similar. Clinicians may improve the cure rate by considering microorganism infection types.
