安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2015年
2期
253-255
,共3页
邓鹏程%徐培坤%朱立新%王斌%杨成子
鄧鵬程%徐培坤%硃立新%王斌%楊成子
산붕정%서배곤%주립신%왕빈%양성자
胶质瘤%甲基化%p16%血浆%癌组织
膠質瘤%甲基化%p16%血漿%癌組織
효질류%갑기화%p16%혈장%암조직
glioma%methylation%p16%plasma%tumor tissue
选择48例脑胶质瘤患者血浆及癌组织作为实验组,10例脑外伤患者坏死脑组织、血浆及10例健康者血浆作为对照组。应用甲基化特异性聚合酶链反应( MSP)和亚硫酸氢盐处理后测序( BSP)法检测p16基因甲基化,显示实验组外周血浆、肿瘤组织中 p16基因甲基化率分别为37.5%(18/48)、43.8%(21/48),对照组均未发现甲基化现象。实验组基因甲基化与临床资料差异无统计学意义。
選擇48例腦膠質瘤患者血漿及癌組織作為實驗組,10例腦外傷患者壞死腦組織、血漿及10例健康者血漿作為對照組。應用甲基化特異性聚閤酶鏈反應( MSP)和亞硫痠氫鹽處理後測序( BSP)法檢測p16基因甲基化,顯示實驗組外週血漿、腫瘤組織中 p16基因甲基化率分彆為37.5%(18/48)、43.8%(21/48),對照組均未髮現甲基化現象。實驗組基因甲基化與臨床資料差異無統計學意義。
선택48례뇌효질류환자혈장급암조직작위실험조,10례뇌외상환자배사뇌조직、혈장급10례건강자혈장작위대조조。응용갑기화특이성취합매련반응( MSP)화아류산경염처리후측서( BSP)법검측p16기인갑기화,현시실험조외주혈장、종류조직중 p16기인갑기화솔분별위37.5%(18/48)、43.8%(21/48),대조조균미발현갑기화현상。실험조기인갑기화여림상자료차이무통계학의의。
The plasma and cancer tissue of the 48 patients for glioma were selected as the experimental group. The necrotic brain tissue and plasma of the 10 patients with traumatic brain injuries were selected as the control group, and the plasma of the 10 healthy people was also selected as the control group. The p16 gene methylation was exam-inated in samples by methylation specific polymerase chain reaction ( MSP) method and bisulfite sequencing after processing (BSP) method. The result shows that the rate of p16 gene methylation in tumor tissues was 43. 8%(21/48) and in gliomas, plasma was 37. 5% (18/48). The methylation phenomenon was not found in the control group. The study found that the p16 gene methylation was no significant correlation with clinical data.