CAJ | 학술논문

目的：探究青春期低剂量氰戊菊酯暴露对小鼠行为的影响。方法对出生后第28天的48只ICR小鼠雌雄各半分别进行不同浓度氰戊菊酯(0、0．02、0．20、2．00 mg/kg )灌胃染毒。出生后第28~56天。在出生后第9周,雌雄小鼠分别进行相关行为学实验。通过黑白巷实验、旷场实验检测小鼠的探索行为和焦虑相关行为,采用Morris水迷宫实验检测小鼠的空间学习和记忆相关行为。结果各组小鼠在旷场实验中的行为差异无统计学意义。在黑白巷实验中,与0 mg/kg组雄性小鼠比较,0．02 mg/kg组和2．00 mg/kg组雄性小鼠跨过中线的潜伏期明显延长(P=0．006,P=0．039)。在Morris水迷宫的定位航行实验中,与0 mg/kg 组雌鼠比较,0．02 mg/kg组和2．00 mg/kg组的雌鼠登陆平台的潜伏期明显延长(P=0．011,P=0．014)；与0 mg/kg组雌鼠比较,0．20 mg/kg 组的雌鼠搜寻平台的距离明显增加( P =0．003)；而雄性小鼠各组指标之间差异无统计学意义。在Morris水迷宫的空间探索实验中,与0 mg/kg组比较,0．20 mg/kg组雌鼠的目标象限时间比降低(P=0．046)；而雄鼠各组指标之间的差异无统计学意义。结论青春期低剂量的氰戊菊酯暴露能够影响小鼠的多种行为,可能影响小鼠青春期的神经行为发育。
목적：탐구청춘기저제량청무국지폭로대소서행위적영향。방법대출생후제28천적48지ICR소서자웅각반분별진행불동농도청무국지(0、0．02、0．20、2．00 mg/kg )관위염독。출생후제28~56천。재출생후제9주,자웅소서분별진행상관행위학실험。통과흑백항실험、광장실험검측소서적탐색행위화초필상관행위,채용Morris수미궁실험검측소서적공간학습화기억상관행위。결과각조소서재광장실험중적행위차이무통계학의의。재흑백항실험중,여0 mg/kg조웅성소서비교,0．02 mg/kg조화2．00 mg/kg조웅성소서과과중선적잠복기명현연장(P=0．006,P=0．039)。재Morris수미궁적정위항행실험중,여0 mg/kg 조자서비교,0．02 mg/kg조화2．00 mg/kg조적자서등륙평태적잠복기명현연장(P=0．011,P=0．014)；여0 mg/kg조자서비교,0．20 mg/kg 조적자서수심평태적거리명현증가( P =0．003)；이웅성소서각조지표지간차이무통계학의의。재Morris수미궁적공간탐색실험중,여0 mg/kg조비교,0．20 mg/kg조자서적목표상한시간비강저(P=0．046)；이웅서각조지표지간적차이무통계학의의。결론청춘기저제량적청무국지폭로능구영향소서적다충행위,가능영향소서청춘기적신경행위발육。
Objective To explore the effects of low dose of fenvalerate exposure during puberty on behaviors in mice. Methods Twenty four male and twenty four female ICR mice in postnatal day 28 were randomly divided into four groups respectively: the control group (0 mg/kg) and fenvalerate group, 0. 02, 0. 20, 2. 00 mg/kg. Mice were treated with corn oil as a vehicle control or fenvalerate by gavage daily from postnatal day 28 to 56 . At the age of 9 weeks, open field, black and white alley, Morris water maze were used to test anxiety, exploratory behavior, spatial learning and memory in mice. Results Exposure to fenvalerate did not alter mice behaviors in open field test. In black and white alley test, pubertal exposure to fenvalerate (0. 02 mg/kg) significantly increased latency in male mice ( P=0. 006 );exposure to fenvalerate ( 2. 00 mg/kg ) significantly increased latency in female mice ( P=0. 039). The results of spatial learning test in Morris water maze showed that pubertal exposure to fenvalerate (0. 20 mg/kg and 2. 00 mg/kg) significantly increased the escape latency of female mice ( P =0. 011, P =0. 014 );exposure to fenvalerate ( 0. 20 mg/kg ) significantly extended the escape distance in the female ( P =0. 003 ) . Spatial memory test results showed that the time proportion of objective quadrant was significantly de-creased in female mice treated with 0. 20 mg/kg ( P=0. 046 ) . Exposure to fenvalerate did not alter behaviors of male mice in Morris water maze test. Conclusion Pubertal exposure to low dose fenvalerate disrupts multiple be-haviors and may affect neurobehavioral development in mice.