CAJ | 학술논문

目的：探讨受体相互作用蛋白1( RIP1)增强顺铂( DDP)诱导食管癌细胞凋亡的敏感性。方法单溶液细胞增殖分析( MTS )法检测不同浓度 DDP 对食管癌细胞株KYSE510、KYSE410的增殖抑制作用；Annexin V/PI 双染流式细胞术检测细胞凋亡；Western blot法检测RIP1、半胱天冬氨酸蛋白酶3(caspase-3)、PARP的蛋白表达。结果 DDP诱导食管癌细胞凋亡具有剂量和时间相关性。凋亡率随剂量增加和时间延长升高， RIP1蛋白的表达升高， DDP 联合RIP1特异性抑制剂处理食管癌细胞后，较敏感的KYSE510凋亡率明显减少。结论 RIP1可能参与了DDP诱导食管癌细胞凋亡的作用。
목적：탐토수체상호작용단백1( RIP1)증강순박( DDP)유도식관암세포조망적민감성。방법단용액세포증식분석( MTS )법검측불동농도 DDP 대식관암세포주KYSE510、KYSE410적증식억제작용；Annexin V/PI 쌍염류식세포술검측세포조망；Western blot법검측RIP1、반광천동안산단백매3(caspase-3)、PARP적단백표체。결과 DDP유도식관암세포조망구유제량화시간상관성。조망솔수제량증가화시간연장승고， RIP1단백적표체승고， DDP 연합RIP1특이성억제제처리식관암세포후，교민감적KYSE510조망솔명현감소。결론 RIP1가능삼여료DDP유도식관암세포조망적작용。
Objective To investigate the role of receptor-interacting protein 1 ( RIP1 ) on the sensitivity of human esophageal squamous carcinoma cells to cisplatin ( DDP )-induced apoptosis and explore a new target for clinical treatment of esophageal squamous carcinoma. Methods The viability of human esophageal squamous carcinoma cell lines KYSE510 and KYSE410 exposed to different concentrations of DDP were detected by CellTiter 96 ? AQue-ous One Solution Cell Proliferation Assay( MTS) assay. Annexin V/PI staining was used to observe cell apoptosis in KYSE510 and KYSE410 cells. Western blot was used to detect RIP1,caspase-3,PARP expression in KYSE510 and KYSE410 cells exposed to DDP. Results DDP induced apoptosis in esophageal squamous carcinoma cells with dose and time dependency. Apoptotic rate was increased with dose and time, RIP1 expression was upregulated in e-sophageal squamous carcinoma cell lines. It was significantly reduced after exposure to DDP association special in-hibitor of RIP1 in KYSE510 cells which were more sensitive to DDP than KYSE410 cells. Conclusion RIP1 may-be participates in the apoptosis of human esophageal squamous carcinoma cells to DDP,suggesting the potential of RIP1 as a new candidate target for clinical treatment of esophageal squamous carcinoma.