CAJ | 학술논문

目的：寻找在中国人群中具有高敏感性、高特异性的膀胱癌甲基化标志物，以应用于膀胱癌的尿液诊断。方法先用T24细胞系筛选8种文献报道的膀胱癌甲基化标志物，再利用甲基化特异性荧光定量PCR(qMSP)技术分别在28例膀胱癌患者、10例尿路结石伴感染患者和30例健康志愿者尿液样品中检测筛选成功的4种甲基化标志物，计算出不同组合的敏感性和特异性。结果 PCDH17、POU4F2、TCF21、ZNF154的敏感性分别为46．43%、92．86%、39．29%、46．43%，特异性分别为95．00%、97．50%、97．50%、100．00%，最优的诊断标志物为 POU4F2(敏感性92．86%、特异性97．50%)。结论应用qMSP技术，以POU4F2为甲基化标志物在尿液中检测膀胱癌可能会成为一种临床可用的诊断方式。
목적：심조재중국인군중구유고민감성、고특이성적방광암갑기화표지물，이응용우방광암적뇨액진단。방법선용T24세포계사선8충문헌보도적방광암갑기화표지물，재이용갑기화특이성형광정량PCR(qMSP)기술분별재28례방광암환자、10례뇨로결석반감염환자화30례건강지원자뇨액양품중검측사선성공적4충갑기화표지물，계산출불동조합적민감성화특이성。결과 PCDH17、POU4F2、TCF21、ZNF154적민감성분별위46．43%、92．86%、39．29%、46．43%，특이성분별위95．00%、97．50%、97．50%、100．00%，최우적진단표지물위 POU4F2(민감성92．86%、특이성97．50%)。결론응용qMSP기술，이POU4F2위갑기화표지물재뇨액중검측방광암가능회성위일충림상가용적진단방식。
Objective To identify a panel of novel epigenetic biomarkers with high sensitivity and specificity that can be utilized in detection and diagnosis of bladder cancer using urine sediments. Methods T24 cell lines that had been treated with bisulfite were used to examine the 8 methylated candidates that were previously reported in bladder cancer patients. Methylation levels of the candidate genes were quantified using the urine sediments from 28 bladder cancer patients, 30 healthy volunteers and 10 infected urinary calculi patients by quantitative methylation-specific polymerase chain reaction(qMSP). The four most efficacious and reliable biomarkers were selected after the sensitivity and specificity of each biomarker were further calculated and inspected. Results The sensitivities of PCDH17, POU4F2, TCF21 and ZNF154 in the detection of bladder cancer were 46. 43%, 92. 86%, 39. 29% and 46. 43% respectively;the specificities of these biomarkers were 95. 00%, 97. 50%, 97. 50% and 100. 00% re-spectively. POU4F2 appeared as the best biomarkers among the four, showing a sensitivity of 92. 86% and a speci-ficity of 97 . 50%. Conclusion Bladder cancer can be detected by a biomarker panel by using qMSP depending on the urine samples from patients.