CAJ | 학술논문

以6个甘蓝杂交种为试材，采用游离小孢子培养方法，研究影响胚状体发生的因素。结果表明：花蕾长度为3．00～4．00 mm时，有利于获得胚状体；1500 r／min离心、32．5℃热激24 h，最有利于胚状体的诱导；花蕾经低温4℃预处理24 h，可提高胚诱导率；低速振荡培养能够促进胚状体发生；基因型在小孢子胚状体诱导中起着主导作用。
이6개감람잡교충위시재，채용유리소포자배양방법，연구영향배상체발생적인소。결과표명：화뢰장도위3．00～4．00 mm시，유리우획득배상체；1500 r／min리심、32．5℃열격24 h，최유리우배상체적유도；화뢰경저온4℃예처리24 h，가제고배유도솔；저속진탕배양능구촉진배상체발생；기인형재소포자배상체유도중기착주도작용。
Taking 6 cabbage hybrids as experimental materials , the influence factors of somatic embryo-genesis were studied by isolated microspore culture method .The results showed that the flower bud length in the range of 3 .00~4 .00 mm was favorable to obtain embryoid;when centrifugation at the speed of 1 500 r/min and heat shocking at 32.5℃ for 24 hours, it was the most beneficial to the induction of embryoid; when the flower buds were pretreated at 4℃for 24 hours , the embryo induction rate could be improved;low oscillation culture could promote the embryogenesis .Genotype played a dominant role in microspore embryoid induction .