CAJ | 학술논문

以处于休眠和解除休眠时期的芍药芽为试验材料，采用改良CTAB法、Trizol法和EASYspin Plus植物RNA快速提取试剂盒提取其总RNA，通过核酸蛋白仪、琼脂糖凝胶电泳和RT－PCR检测所提总RNA样品的品质。结果表明，Trizol法没有提取到芍药芽总RNA；EASYspin Plus植物RNA快速提取试剂盒提取的总RNA浓度太低，无法满足下一步试验的要求；改良CTAB法提得的总RNA较完整，条带清晰，品质较优，28S rRNA亮度约是18S rRNA的2倍，且无降解现象。以改良的CTAB法提取的总RNA为模板进行内参Actin扩增，得到的条带清晰，与目的片段大小一致，进一步证明此方法提取的总RNA能够满足后续试验的要求。
이처우휴면화해제휴면시기적작약아위시험재료，채용개량CTAB법、Trizol법화EASYspin Plus식물RNA쾌속제취시제합제취기총RNA，통과핵산단백의、경지당응효전영화RT－PCR검측소제총RNA양품적품질。결과표명，Trizol법몰유제취도작약아총RNA；EASYspin Plus식물RNA쾌속제취시제합제취적총RNA농도태저，무법만족하일보시험적요구；개량CTAB법제득적총RNA교완정，조대청석，품질교우，28S rRNA량도약시18S rRNA적2배，차무강해현상。이개량적CTAB법제취적총RNA위모판진행내삼Actin확증，득도적조대청석，여목적편단대소일치，진일보증명차방법제취적총RNA능구만족후속시험적요구。
The total RNA was extracted from dormancy and dormancy -released peony buds by modified CTAB method , Trizol method and EASYspin Plus RNA rapid isolation kit respectively .The quality of the ex-tracted RNA was tested by protein nucleic acid analyzer , agarose gel electrophoresis and RT -PCR.The re-sults showed that the total RNA could not be extracted by Trizol method , and that extracted by EASYspin Plus RNA rapid isolation kit was too lower to meet the requirement of further experiment .The total RNA extracted by modified CTAB method was more complete with clearer bands and better quality , and the brightness of 28 S rRNA was about 2 times of that of 18S rRNA with no degradation .Using the total RNA extracted by modified CTAB method as template to amplify the internal reference gene Actin, the obtained bands were clear and with the same size to the target fragment , which further proved that the total RNA extracted by modified CTAB method could meet the requirement of the later experiment .