山东农业科学
山東農業科學
산동농업과학
SHANGDONG AGRICULTURAL SCIENCES
2015年
2期
12-15
,共4页
周鹏飞%柳絮%张华%宣宁%李军%杨永义%李广贤%姚方印%刘开启
週鵬飛%柳絮%張華%宣寧%李軍%楊永義%李廣賢%姚方印%劉開啟
주붕비%류서%장화%선저%리군%양영의%리엄현%요방인%류개계
水稻%抽穗期%BSA%SSR分子标记%基因定位
水稻%抽穗期%BSA%SSR分子標記%基因定位
수도%추수기%BSA%SSR분자표기%기인정위
Oryza sativa%Heading date%Bulked segregation analysis%SSR molecular marker%Gene location
本试验以明恢63（供体）／早熟豫6（受体）的BC4 F2分离群体为材料，采用BSA （ Bulked segrega-tion analysis）法，对控制水稻抽穗期分离的基因进行SSR分子标记定位。 BC4 F2出现抽穗期分离，早抽穗植株为253株，晚抽穗植株为657株，χ2＝3．66＜P0．05＝3．84，符合1∶3的孟德尔分离比，表明F2群体的抽穗期分离受一对等位基因控制，晚抽穗性状为显性。以明恢63×早熟豫6的BC4 F2分离群体的253株隐性单株为定位群体，将该抽穗基因（暂时命名为Hd6－f1）定位在水稻第6染色体分子标记RM19771与RM527之间，遗传距离分别为0．2 cM和2．9 cM，与RM19780共分离。
本試驗以明恢63（供體）／早熟豫6（受體）的BC4 F2分離群體為材料，採用BSA （ Bulked segrega-tion analysis）法，對控製水稻抽穗期分離的基因進行SSR分子標記定位。 BC4 F2齣現抽穗期分離，早抽穗植株為253株，晚抽穗植株為657株，χ2＝3．66＜P0．05＝3．84，符閤1∶3的孟德爾分離比，錶明F2群體的抽穗期分離受一對等位基因控製，晚抽穗性狀為顯性。以明恢63×早熟豫6的BC4 F2分離群體的253株隱性單株為定位群體，將該抽穗基因（暫時命名為Hd6－f1）定位在水稻第6染色體分子標記RM19771與RM527之間，遺傳距離分彆為0．2 cM和2．9 cM，與RM19780共分離。
본시험이명회63（공체）／조숙예6（수체）적BC4 F2분리군체위재료，채용BSA （ Bulked segrega-tion analysis）법，대공제수도추수기분리적기인진행SSR분자표기정위。 BC4 F2출현추수기분리，조추수식주위253주，만추수식주위657주，χ2＝3．66＜P0．05＝3．84，부합1∶3적맹덕이분리비，표명F2군체적추수기분리수일대등위기인공제，만추수성상위현성。이명회63×조숙예6적BC4 F2분리군체적253주은성단주위정위군체，장해추수기인（잠시명명위Hd6－f1）정위재수도제6염색체분자표기RM19771여RM527지간，유전거리분별위0．2 cM화2．9 cM，여RM19780공분리。
Using the BC4F2 segregation populations of Minghui 63(donor) and Zaoshuyu 6(receptor) as materials, the SSR markers were located for the gene on rice heading date by the bulked segregation analysis (BSA).The BC4F2 population had 253 early heading plants and 657 late heading plants( χ2 =3.66<P0.05 =3 .84 ) .Its segregation accorded with the ratio of 1∶3 , which confirmed that the heading date was controlled by a pair of alleles and the late heading character was dominant .Using the 253 early heading plants of BC 4 F2 population as mapping population , the gene on heading date named Hd6-f1 was mapped on chromosome 6 between SSR markers RM19771 and RM527 with the genetic distances as 0.2 cM and 2.9 cM respectively, and co-segregated with RM19780 .
