CAJ | 학술논문

CCCH型锌指蛋白是一类生物体中广泛存在的转录因子，与植物的抗逆性密切相关。水稻CCCH锌指蛋白家族包括67个成员，分为8个亚家族。本研究采用实时定量PCR技术分析水稻CCCH亚家族Ⅰ基因的组织表达模式以及盐、干旱、低温、高温等多种非生物胁迫和脱落酸（ ABA）对CCCH基因表达水平的调节。结果表明，多数 CCCH 基因都不同程度地受到这些非生物胁迫及 ABA 的诱导或抑制，其中 C3H10、C3H24、C3H33、C3H37和C3H67的表达量在不同胁迫处理后表现出不同程度的升高，C3H35、C3H50和C3H52在盐、干旱、ABA、低温和高温处理后表达水平呈下降趋势，表明这些CCCH锌指蛋白可能参与水稻的非生物胁迫响应。
CCCH형자지단백시일류생물체중엄범존재적전록인자，여식물적항역성밀절상관。수도CCCH자지단백가족포괄67개성원，분위8개아가족。본연구채용실시정량PCR기술분석수도CCCH아가족Ⅰ기인적조직표체모식이급염、간한、저온、고온등다충비생물협박화탈락산（ ABA）대CCCH기인표체수평적조절。결과표명，다수 CCCH 기인도불동정도지수도저사비생물협박급 ABA 적유도혹억제，기중 C3H10、C3H24、C3H33、C3H37화C3H67적표체량재불동협박처리후표현출불동정도적승고，C3H35、C3H50화C3H52재염、간한、ABA、저온화고온처리후표체수평정하강추세，표명저사CCCH자지단백가능삼여수도적비생물협박향응。
CCCH-type zinc finger proteins , a kind of widely distributed transcription factors , are close-ly related to plant tolerance to stress .CCCH-type zinc finger protein family in rice contains 67 members, which are devided into 8 subfamilies .The tissue-specific expression patterns of CCCH -zinc finger protein subfamily Ⅰgenes and the adjustment of ABA and abiotic stresses such as salt , drought , low and hight tem-perature to CCCH genes expression level were analyzed by real time PCR .The results showed that most of CCCH genes were induced or restrained by the abiotic stresses and ABA at varying degrees .The expression quantities of C3H10, C3H24, C3H33, C3H37 and C3H67 genes were up-regulated, while those of C3H35, C3H50 and C3H52 genes were declining .It indicated that CCCH subfamily Ⅰgenes might be involved in re-sponse to abiotic stress .