CAJ | 학술논문

为了解上海地区猪源沙门氏菌的耐药性及基因分型情况，本研究对2013年分离到的13株猪源沙门氏菌（10株为猪霍乱沙门氏菌，3株为鼠伤寒沙门氏菌）采用微量肉汤稀释法进行药物敏感性测定，应用脉冲场凝胶电泳（PFGE）进行基因分型。药物敏感试验结果表明，分离到的13株沙门氏菌对磺胺异恶唑、多西环素、氟苯尼考和阿莫西林的耐药率较高，对阿莫西林/棒酸、头孢曲松、丁胺卡那霉素和头孢噻呋敏感。脉冲场凝胶电泳分型结果显示，10株猪霍乱沙门氏菌中有8株为流行病学相关，是本次研究中猪霍乱沙门氏菌的优势基因型。
위료해상해지구저원사문씨균적내약성급기인분형정황，본연구대2013년분리도적13주저원사문씨균（10주위저곽란사문씨균，3주위서상한사문씨균）채용미량육탕희석법진행약물민감성측정，응용맥충장응효전영（PFGE）진행기인분형。약물민감시험결과표명，분리도적13주사문씨균대광알이악서、다서배소、불분니고화아막서림적내약솔교고，대아막서림/봉산、두포곡송、정알잡나매소화두포새부민감。맥충장응효전영분형결과현시，10주저곽란사문씨균중유8주위류행병학상관，시본차연구중저곽란사문씨균적우세기인형。
To investigate the drug-resistance and genotype of Salmonella from pig in Shanghai,13 Salmonella isolates(10 Salmonella choleraesuis,3 Salmonella typhimurium)were tested for their drug-resistance using broth dilution method,and genotyped by pulsed-field gel electrophoresis(PFGE). Drug-resistance test showed that the 13 Salmonella isolates were resistant to sulfisoxazole,doxycycline,florfenicol and amoxicillin,and sensitive to amoxicillin/clavulanic acid,ceftriaxone,amikacin and ceftiofur. Pulsed-field gel electrophoresis typing showed that 8 Salmonella choleraesuis were epidemiologically linked,and were the dominant genotype in our study.