CAJ | 학술논문

[目的]比较IHA和ELISA 2种基层使用较广泛的猪瘟抗体检测试剂盒检测结果的一致性。[方法]用产品A（IHA）和产品B（ELISA）对237份猪血清进行抗体检测，分别计算阳性率、阳性一致性、阴性一致性、符合率，并进行Kappa检验。[结果]产品B检测的阳性率（73.8%）高于A（67.1%），且有统计学意义（p<0.01）。2种试剂盒共同检出128份猪瘟抗体阳性和31份阴性，阳性一致性为80.5%（95%置信区间：74.3%-86.7%），阴性一致性为39.7%（95%置信区间：28.9%-50.6%），符合率67.1%（95%置信区间：61.1%-73.1%）；Kappa值为0.214（95%置信区间：0.088-0.339）。[结论]2种试剂盒的检测结果阳性率有差异，结果一致性强度为弱。
[목적]비교IHA화ELISA 2충기층사용교엄범적저온항체검측시제합검측결과적일치성。[방법]용산품A（IHA）화산품B（ELISA）대237빈저혈청진행항체검측，분별계산양성솔、양성일치성、음성일치성、부합솔，병진행Kappa검험。[결과]산품B검측적양성솔（73.8%）고우A（67.1%），차유통계학의의（p<0.01）。2충시제합공동검출128빈저온항체양성화31빈음성，양성일치성위80.5%（95%치신구간：74.3%-86.7%），음성일치성위39.7%（95%치신구간：28.9%-50.6%），부합솔67.1%（95%치신구간：61.1%-73.1%）；Kappa치위0.214（95%치신구간：0.088-0.339）。[결론]2충시제합적검측결과양성솔유차이，결과일치성강도위약。
Objective] To compare the consistency between the 2 CSFV antibody kits(IHA and ELISA)widely used in veterinary labs at grassroots level in China. [Method] 237 swine serum samples were tested by kit A(IHA)and kit B(ELISA). The positive rates,positive and negative consistence rates,coincidence rate were calculated separate-ly,and the consistency was evaluated by Kappa test. [Results] The positive rate of kit B(73.8%)was higher than kit A(67.1%)and there was a statistically significant difference(p<0.01).128 positive and 31 negative were detected by the 2 kits together,the positive consistency rate was 80.5%(95%CI=74.3%-86.7%)and the negative consistency rate was 39.7%(95%CI=28.9%-50.6%),the coincidence rate was 67.1%(95%CI=61.1%-73.1%).The Kappa value was 0.214 (95%CI=0.088-0.339). [Conclusion] The results showed that there was a significant difference between the two test-ing positive rates and a weak consistency between the two kits.