CAJ | 학술논문

为了解猪博卡病毒（PBoV）的遗传进化规律，研究可行的PBoV基因分型方法，本研究利用GenBank中登录的29条PBoV和2条本实验室测得的全基因组序列信息，采用生物信息学软件进行基因组序列分析。结果显示，分别以NS1基因、NP1基因和VP1基因编码氨基酸构建的系统进化树，PBoV均被划分为3个基因群，但三种氨基酸序列绘制的进化树存在差异；基因群内各病毒株的氨基酸序列同源性较高，而各基因群之间病毒株的氨基酸序列同源性非常低，基因组结构差异较大。
위료해저박잡병독（PBoV）적유전진화규률，연구가행적PBoV기인분형방법，본연구이용GenBank중등록적29조PBoV화2조본실험실측득적전기인조서렬신식，채용생물신식학연건진행기인조서렬분석。결과현시，분별이NS1기인、NP1기인화VP1기인편마안기산구건적계통진화수，PBoV균피화분위3개기인군，단삼충안기산서렬회제적진화수존재차이；기인군내각병독주적안기산서렬동원성교고，이각기인군지간병독주적안기산서렬동원성비상저，기인조결구차이교대。
To analyze the genomic characteristics and classify the genotypes of porcine Bocavirus(PBoV),the complete genomic sequences of 29 PBoVs available in GenBank and 2 PboVs detected by our laboratory were analyzed for the homology of NS1,NP1,VP1 amino acid sequences by bioinformatics software. The results showed that PBoV could be divided into three groups based on phylogenetic tree established according to PBoV NS1,NP1,VP1 amino acid sequences,but there were also some noticeable differences among them:the viruses in the same group shared higher sequence identity and those in different groups shared lower sequence identity.