CAJ | 학술논문

本研究针对猪繁殖与呼吸综合征病毒美洲型ORF6基因的6个区域，利用2对引物建立检测猪繁殖与呼吸综合征病毒的RT-LAMP快速检测方法，该方法具有高灵敏度，高特异性的优点，在2小时左右即可得出结果，其灵敏度与PRRSV荧光RT-PCR检测方法相近，高于普通RT-PCR检测方法。将灭活的猪繁殖与呼吸综合征病毒美洲型培养物作10倍系列稀释，结果显示建立的荧光RT-PCR的检测极限可达10-7，RT-PCR检测方法的检测极限为10-5，RT-LAMP检测方法达到10-8，表明建立的RT-LAMP检测方法非常灵敏，可用于检测猪组织样本中的美洲型猪繁殖与呼吸综合征病毒。
본연구침대저번식여호흡종합정병독미주형ORF6기인적6개구역，이용2대인물건립검측저번식여호흡종합정병독적RT-LAMP쾌속검측방법，해방법구유고령민도，고특이성적우점，재2소시좌우즉가득출결과，기령민도여PRRSV형광RT-PCR검측방법상근，고우보통RT-PCR검측방법。장멸활적저번식여호흡종합정병독미주형배양물작10배계렬희석，결과현시건립적형광RT-PCR적검측겁한가체10-7，RT-PCR검측방법적검측겁한위10-5，RT-LAMP검측방법체도10-8，표명건립적RT-LAMP검측방법비상령민，가용우검측저조직양본중적미주형저번식여호흡종합정병독。
Two pairs of primers targeting six regions of American-type porcine reproductive and respiratory syndrome virus ORF6 gene,and RT-LAMP assay was established for the rapid detection of porcine reproductive and respiratory virus with high sensitivity and specificity. It could be finished in about 2 hours. The sensitivity of the assay was similar to PRRSV fluorescent RT-PCR,and higher than the normal RT-PCR. The inactivated porcine reproductive and respiratory syndrome virus culture was serially diluted in 10-folds,and tested with PRRSV fluorescent RT-PCR,RT-LAMP assay and normal RT-PCR. The result showed that the fluorescence RT-PCR detection limit was up to 10-7,the normal RT-PCR detection limit was 10-5,and the RT-LAMP detection limit was 10-8,indicating RT-LAMP assay was very sensitive. The assay could be used for rapid detection of the American-type porcine reproductive and respiratory syndrome virus in various pig tissue samples.