山东农业科学
山東農業科學
산동농업과학
SHANGDONG AGRICULTURAL SCIENCES
2015年
2期
58-60
,共3页
孙淑琴%杨秀荣%田涛%孙冰冰
孫淑琴%楊秀榮%田濤%孫冰冰
손숙금%양수영%전도%손빙빙
黄瓜白粉病%拮抗细菌筛选%解淀粉芽孢杆菌
黃瓜白粉病%拮抗細菌篩選%解澱粉芽孢桿菌
황과백분병%길항세균사선%해정분아포간균
Cucumber powdery mildew%Screening of antagonistic bacteria%Bacillus amyloliquefaciens
为筛选对黄瓜白粉病菌具有拮抗作用的生防菌株,从温室土壤样品中分离到细菌菌株692株。经室内平板拮抗性筛选和盆栽防治试验,筛选出菌株Bs-18对黄瓜白粉病具有良好的防治作用,盆栽防效达82.22%。对菌株Bs-18进行形态特征、生理生化特性和16 S rDNA测序分析,将其鉴定为解淀粉芽孢杆菌( Bacillus amyloliquefaciens)。
為篩選對黃瓜白粉病菌具有拮抗作用的生防菌株,從溫室土壤樣品中分離到細菌菌株692株。經室內平闆拮抗性篩選和盆栽防治試驗,篩選齣菌株Bs-18對黃瓜白粉病具有良好的防治作用,盆栽防效達82.22%。對菌株Bs-18進行形態特徵、生理生化特性和16 S rDNA測序分析,將其鑒定為解澱粉芽孢桿菌( Bacillus amyloliquefaciens)。
위사선대황과백분병균구유길항작용적생방균주,종온실토양양품중분리도세균균주692주。경실내평판길항성사선화분재방치시험,사선출균주Bs-18대황과백분병구유량호적방치작용,분재방효체82.22%。대균주Bs-18진행형태특정、생리생화특성화16 S rDNA측서분석,장기감정위해정분아포간균( Bacillus amyloliquefaciens)。
To obtain antagonistic bacteria for biocontrol of cucumber powdery mildew , 692 bacterial strains were isolated from different greenhouse soil samples .The strain Bs-18 , which had good inhibitory effect on cucumber powdery mildew with the control effect of 82 .22%, was screened out using plate confrontation method and pot test .Based on the morphological characteristics , physicochemical properties and 16 S rDNA sequence analysis , the strain Bs-18 was identified as Bacillus amyloliquefaciens.