CAJ | 학술논문

分别采用木瓜蛋白酶和溶壁酶降解金针菇菇根水提后的多糖，以提取还原性寡糖，并通过单因素试验和正交试验确定适合两种酶的最佳提取工艺。结果表明，木瓜蛋白酶降解菇根多糖的最佳工艺条件为酶解0．5 h、酶底比为4．5％、pH值6．0、温度为35℃；溶壁酶的最佳工艺条件为酶解1．5 h、酶底比为2．0％、pH值5．0、温度为50℃。采用不同的酶降解金针菇多糖，其还原性寡糖的得率不同，其中，溶壁酶降解所得还原性寡糖得率为6．8768％，木瓜蛋白酶降解所得还原性寡糖得率为6．1368％。
분별채용목과단백매화용벽매강해금침고고근수제후적다당，이제취환원성과당，병통과단인소시험화정교시험학정괄합량충매적최가제취공예。결과표명，목과단백매강해고근다당적최가공예조건위매해0．5 h、매저비위4．5％、pH치6．0、온도위35℃；용벽매적최가공예조건위매해1．5 h、매저비위2．0％、pH치5．0、온도위50℃。채용불동적매강해금침고다당，기환원성과당적득솔불동，기중，용벽매강해소득환원성과당득솔위6．8768％，목과단백매강해소득환원성과당득솔위6．1368％。
In order to obtain oligosaccharides , the papain and lywallzyme were used respectively to de-grade the polysaccharides extracted from enoki mushroom root with water .The optimum enzymolysis conditions for two enzymes were optimized through single -factor experiment and orthogonal test .The results showed that the optimum conditions for papain were enzymolysis for 0.5 hours, the ratio of enzyme to substrate as 4.5%, pH 6.0 and the temperature as 35℃.The optimum conditions for lywallzyme were enzymolysis for 1.5 hours, the ratio of enzyme to substrate as 2.0%, pH 5.0 and the temperature as 50℃.Using different enzymes to degrade enoki mushroom polysaccharides , the production of oligosaccharides was different .The yield of reduc-ing oligosaccharide obtained by lywallzyme and papain were 6.8768%and 6.1368%respectively.