中国医科大学学报
中國醫科大學學報
중국의과대학학보
JOURNAL OF CHINA MEDICAL UNIVERSITY
2015年
2期
105-108
,共4页
增食欲素A%vaspin%胰岛素抵抗%肥胖
增食欲素A%vaspin%胰島素牴抗%肥胖
증식욕소A%vaspin%이도소저항%비반
orexin A%vaspin%insulin resistance%obesity
目的：分析胰岛素抵抗（IR）肥胖大鼠体内增食欲素A（orexin A）与脂肪组织特异性丝氨酸蛋白酶抑制剂（vaspin）的相关性，阐明IR病理过程中orexin参与脂肪代谢的可能作用机制。方法4周龄SD雄性大鼠75只，随机分为正常饮食（NC）组（n=20）和高脂肪膳食（HF）组（n=55），建立IR肥胖大鼠模型，钳夹技术评定IR及脂代谢相关指标。大鼠皮下注射orexin A（1×10-8~1×10-6 mol/L），用酶联免疫吸附法检测血清orexin A和vaspin。结果（1）喂养6周后，与NC组比较，HF组大鼠血糖、胰岛素、TC、TG、LDL?C均增高（P均<0.05）；与NC组比较，GIR60~120降低[（16.31±1.54）vs（30.22±2.76）mg/（kg·min），P<0.05]；（2）HF组较NC组血清vaspin水平增加177.08%（P<0.05），NC组皮下注射orexin A（1×10-8，1×10-7，1×10-6 mol/L），NC组大鼠血清vaspin分别增加25.00%、68.75%、120.83%，HF组大鼠血清vaspin分别增加7.52%、24.06%、40.60%；（3）IR肥胖大鼠vaspin与orexin A、血糖、胰岛素、TC、TG、LDL?C呈正相关（r分别为0.482、0.515、0.303、0.388、0.255、0.253，P=0.04），与HDL?C呈负相关（r=-0.226，P均<0.05）。结论高脂膳食诱导的IR肥胖大鼠机体内orexin A与vaspin密切相关，orexin A增强vaspin的表达，进而参与肥胖大鼠机体内IR的发生。
目的：分析胰島素牴抗（IR）肥胖大鼠體內增食欲素A（orexin A）與脂肪組織特異性絲氨痠蛋白酶抑製劑（vaspin）的相關性，闡明IR病理過程中orexin參與脂肪代謝的可能作用機製。方法4週齡SD雄性大鼠75隻，隨機分為正常飲食（NC）組（n=20）和高脂肪膳食（HF）組（n=55），建立IR肥胖大鼠模型，鉗夾技術評定IR及脂代謝相關指標。大鼠皮下註射orexin A（1×10-8~1×10-6 mol/L），用酶聯免疫吸附法檢測血清orexin A和vaspin。結果（1）餵養6週後，與NC組比較，HF組大鼠血糖、胰島素、TC、TG、LDL?C均增高（P均<0.05）；與NC組比較，GIR60~120降低[（16.31±1.54）vs（30.22±2.76）mg/（kg·min），P<0.05]；（2）HF組較NC組血清vaspin水平增加177.08%（P<0.05），NC組皮下註射orexin A（1×10-8，1×10-7，1×10-6 mol/L），NC組大鼠血清vaspin分彆增加25.00%、68.75%、120.83%，HF組大鼠血清vaspin分彆增加7.52%、24.06%、40.60%；（3）IR肥胖大鼠vaspin與orexin A、血糖、胰島素、TC、TG、LDL?C呈正相關（r分彆為0.482、0.515、0.303、0.388、0.255、0.253，P=0.04），與HDL?C呈負相關（r=-0.226，P均<0.05）。結論高脂膳食誘導的IR肥胖大鼠機體內orexin A與vaspin密切相關，orexin A增彊vaspin的錶達，進而參與肥胖大鼠機體內IR的髮生。
목적：분석이도소저항（IR）비반대서체내증식욕소A（orexin A）여지방조직특이성사안산단백매억제제（vaspin）적상관성，천명IR병리과정중orexin삼여지방대사적가능작용궤제。방법4주령SD웅성대서75지，수궤분위정상음식（NC）조（n=20）화고지방선식（HF）조（n=55），건립IR비반대서모형，겸협기술평정IR급지대사상관지표。대서피하주사orexin A（1×10-8~1×10-6 mol/L），용매련면역흡부법검측혈청orexin A화vaspin。결과（1）위양6주후，여NC조비교，HF조대서혈당、이도소、TC、TG、LDL?C균증고（P균<0.05）；여NC조비교，GIR60~120강저[（16.31±1.54）vs（30.22±2.76）mg/（kg·min），P<0.05]；（2）HF조교NC조혈청vaspin수평증가177.08%（P<0.05），NC조피하주사orexin A（1×10-8，1×10-7，1×10-6 mol/L），NC조대서혈청vaspin분별증가25.00%、68.75%、120.83%，HF조대서혈청vaspin분별증가7.52%、24.06%、40.60%；（3）IR비반대서vaspin여orexin A、혈당、이도소、TC、TG、LDL?C정정상관（r분별위0.482、0.515、0.303、0.388、0.255、0.253，P=0.04），여HDL?C정부상관（r=-0.226，P균<0.05）。결론고지선식유도적IR비반대서궤체내orexin A여vaspin밀절상관，orexin A증강vaspin적표체，진이삼여비반대서궤체내IR적발생。
Objective To investigate the correlation between orexin A and the serine proteinase inhibitor vaspin in obese rats with insulin resis?tance(IR)induced by high?fat diet and elaborate the possible action mechanism of orexin involvement in fat metabolism in IR pathological process. Methods A total of 75 4?week?old male Sprague?Dawley rats were randomly divided into the normal dietary group(NC group,n=20)and the high?fat dietary group(HF group,n=55)to establish the model of obese rats with IR,the euglycemic insulin clamp technique was used to determine re?lated indicators of insulin resistance and lipid metabolism. The rats were treated with orexin A(1×10-8?1×10-6 mol/L)by hypodermic injection. The serum levels of orexin A and vaspin in rats were detected with enzyme linked immunosorbent assay. Results After 6 weeks of high?fat diet,the se?rum glucose,insulin,TC,TG and LDL?C were increased significantly in HF group than in NC group,while GIR60~120 in obese rats was decreased significantly[(16.31 ± 1.54)vs(30.22 ± 2.76)mg/(kg · min),P<0.05]. The serum vaspin level was increased 177.08%in HF group compared with NC group(P<0.05). With hypodermic injection of orexin A(1×10-8 mol/L,1×10-7 mol/L and 1×10-6 mol/L),the levels of serum vaspin in?creased 25.00%,68.75%,and 120.83%in NC group and increased 7.52%,24.06%,and 40.60%in HF group. There was a positive correlation be?tween vaspin and orexin A,glucose,insulin,TC,TG,and LDL?C(r1=0.482,P1=0.02,r2=0.515,P2=0.02,r3=0.303,P3=0.04,r4=0.388,P4=0.03,r5=0.255,P5=0.04,r6=0.253,P6=0.04)and a negative correlation between vaspin and HDL?C(r=-0.226,P=0.04)in obese rats with IR. Conclusion High?fat diet can induce insulin resistance and obesity in rats,and orexin A is closely correlated to vaspin in obese rats with insu?lin resistance. Orexin A increases serum vaspin expression and thus involves in onset of insulin resistance in obese rats.
