CAJ | 학술논문

为了更准确、高效地利用分子标记辅助选择技术进行辣椒细胞质雄性不育育性基因的选择，本试验利用36份已知基因型（RfRf或rfrf）的辣椒自交系对11对已报道的辣椒恢复基因连锁标记进行适用性检验。结果表明，显性标记CRF3S1S和CRF-SCAR的适用性最广，其准确率分别为91.67%、88.89%；在6对共显性标记中CaRf-FL-M2的适用性最广，其准确率为80.56%。育种实际应用中可以利用标记CRF3S1S或CRF-SCAR对辣椒材料进行初次鉴定，对其中含有Rf基因的辣椒材料利用标记CaRf-FL-M2进行再次鉴定。
위료경준학、고효지이용분자표기보조선택기술진행랄초세포질웅성불육육성기인적선택，본시험이용36빈이지기인형（RfRf혹rfrf）적랄초자교계대11대이보도적랄초회복기인련쇄표기진행괄용성검험。결과표명，현성표기CRF3S1S화CRF-SCAR적괄용성최엄，기준학솔분별위91.67%、88.89%；재6대공현성표기중CaRf-FL-M2적괄용성최엄，기준학솔위80.56%。육충실제응용중가이이용표기CRF3S1S혹CRF-SCAR대랄초재료진행초차감정，대기중함유Rf기인적랄초재료이용표기CaRf-FL-M2진행재차감정。
In order to accurately and efficiently use molecular marker assisted selection technique in selecting Restorer-of-fertility gene in pepper(Capsicum annuum L.)lines,this experiment carried out applicability test on the 11 pairs of reported Restorer-of-fertility gene linked markers with 36 pepper inbred line,whose genotype (RfRf or rfrf)has been known. The results indicated that dominant markers CRF3S1S and CRF-SCAR had the broadest applicability,and their accuracy rate were 91.67% and 88.89%,respectively. CaRf-FL-M2 had the broadest applicability among the 6 pairs of co-dominant markers,and its accuracy rate was 80.56%. In breeding practice,CRF3S1S or CRF-SCAR markers could be used to preliminarily identify pepper materials. Then one more identification could be conducted on pepper material containing Rf gene by CaRf-FL-M2 marker.