CAJ | 학술논문

目的：建立克霉唑阴道片中克霉唑含量的测定方法。方法采用高效液相色谱法，以HYPERSIL ODS C18柱（Φ4.6 mm×250.0 mm），粒度5μm为分析柱，磷酸氢二钾溶液（取磷酸氢二钾4.35 g，加水溶解并稀释至1000 ml）-甲醇（1：3）为流动相，流速为1.0 ml/min，检测波长为254 nm。结果克霉唑在0.1～5.0μg/ml范围内具有良好的线性关系。r=0.9999，平均回收率为99.0%。结论本测定方法简便，准确，可有效测定克霉唑阴道片中克霉唑的含量。
목적：건립극매서음도편중극매서함량적측정방법。방법채용고효액상색보법，이HYPERSIL ODS C18주（Φ4.6 mm×250.0 mm），립도5μm위분석주，린산경이갑용액（취린산경이갑4.35 g，가수용해병희석지1000 ml）-갑순（1：3）위류동상，류속위1.0 ml/min，검측파장위254 nm。결과극매서재0.1～5.0μg/ml범위내구유량호적선성관계。r=0.9999，평균회수솔위99.0%。결론본측정방법간편，준학，가유효측정극매서음도편중극매서적함량。
Objective To establish a method for determination of clotrimazole clotrimazole vaginal tablets content. Methods Using high performance liquid chromatography with ODS-C18 column,HYPERSIL(phi 4.6 mm×250.0 mm), particle size of 5 mu m as analytical column,the solution of potassium hydrogen phosphate two(take two 4.35 G potassium hydrogen phosphate,dissolved in water and diluted to 1 000 ml)-methanol(1:3)as mobile phase,the flow rate is 1 ml/min, the detection wavelength was 254 nm.Results Clotrimazole in 0.1~5.0μg/ml range has a good linear relationship. r=0.9999,the average recovery was 99.0%.Conclusion This method was simple,accurate the content can efectively determine the Clotrimazole Vaginal Tablets in clotrimazole.